Screening for Mimotopes for the Peanut Allergen Ara h 5 Using a Phage Display Random Heptapeptide Library

doi:10.7506/spkx1002-6630-20251018-096

FOOD SCIENCE ›› 2026, Vol. 47 ›› Issue (7): 101-108.doi: 10.7506/spkx1002-6630-20251018-096

• Bioengineering • Previous Articles Next Articles

Screening for Mimotopes for the Peanut Allergen Ara h 5 Using a Phage Display Random Heptapeptide Library

WANG Junjuan, QIN Peiyou, WANG Dan, ZHAO Yuanyuan, PENG Yu, ZHAO Xiaoyan, CHE Huilian

(1. Beijing Key Laboratory of Fruits and Vegetable Storage and Processing, Key Laboratory of Vegetable Postharvest Processing, Ministry of Agriculture and Rural Affairs, Institute of Agri-food Processing and Nutrition, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; 2. College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China)

Online:2026-04-15 Published:2026-05-08

Abstract

Abstract: This study aimed to screen for mimotopes for the peanut allergen Ara h 5 using a phage display random heptapeptide library. Recombinant Ara h 5, obtained via a prokaryotic expression system, was used as an immunogen to generate antibodies in a mouse model. Polyclonal IgE antibodies against Ara h 5 were purified from the antiserum by affinity chromatography. Using these Ara h 5 IgE antibodies as the target, three rounds of biopanning were performed against the phage display heptapeptide library. Positive phage clones were isolated and sequenced. Three mimotope sequences, FHWWYLK, WETIYSR, and GPLWNVN, were identified. These mimotopes provide an important foundation for the development of blocking antibodies or epitope-specific immunotherapy for peanut allergy.

Key words: peanut allergy; Ara h 5; phage display technology; mimotope

CLC Number:

TS201.6

WANG Junjuan, QIN Peiyou, WANG Dan, ZHAO Yuanyuan, PENG Yu, ZHAO Xiaoyan, CHE Huilian. Screening for Mimotopes for the Peanut Allergen Ara h 5 Using a Phage Display Random Heptapeptide Library[J]. FOOD SCIENCE, 2026, 47(7): 101-108.

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Screening for Mimotopes for the Peanut Allergen Ara h 5 Using a Phage Display Random Heptapeptide Library

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