Optimization of Microwave-assisted Extraction of Saponins from the Whole Plant of Alternant heraphiloxeroides (Mart.) Griseb

doi:10.7506/spkx1002-6630-201102020

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (2 ): 84-87.doi: 10.7506/spkx1002-6630-201102020

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Optimization of Microwave-assisted Extraction of Saponins from the Whole Plant of Alternant heraphiloxeroides (Mart.) Griseb

CAI Ling-yun1,2，LI Yun-xiang2，HE Shun-mei2，PAN Qi-bing2，SU Yuan-rong1

1. School of Environmental and Life Science, Kaili University, Kaili 556011, China；2. Key Laboratory for Southwest Wildlife
Resouces Conservation, Minstry of Education, College of Life Science, China West Normal University, Nanchong 637002, China

Received:2010-03-17 Online:2011-01-25 Published:2011-01-10
Contact: CAI Ling-yun1 E-mail:cailingyun2005@sina.com

Abstract

Abstract:

Objective: The development of an optimal process for the microwave-assisted extraction of saponins from the whole plant of Alteman theraphiloxeroides (Mart.) Griseb (APG) in presented in this paper. Methods: Microwave was used for the extraction of saponins with aqueous methanol solution as an assistant approach, and process optimization was achieved by single factor and orthogonal array design experiments. The quantitative analysis of saponins was performed using oleanolic acid as a reference substance, which showed maximum adsorption at 570 nm wavelength, with a calibration equation of Y = 1.904C ＋0.0054 (r = 0.9990). Results: The optimum process conditions for the extraction of saponins were 20 s microwave treatment at 640 W power for intermittently extracting with a 20-fold volume of 20% aqueous solution for 4 times. Conclusion: The developed extraction process is easy to operate and reliable.

Key words: Alteman theraphiloxeroides (Mart.) Griseb (APG), saponin, microwave-assisted extraction

CLC Number:

R284.2

CAI Ling-yun1,2，LI Yun-xiang2，HE Shun-mei2，PAN Qi-bing2，SU Yuan-rong1. Optimization of Microwave-assisted Extraction of Saponins from the Whole Plant of Alternant heraphiloxeroides (Mart.) Griseb[J]. FOOD SCIENCE, 2011, 32(2 ): 84-87.

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Optimization of Microwave-assisted Extraction of Saponins from the Whole Plant of Alternant heraphiloxeroides (Mart.) Griseb

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