Abstract:

The polyphenol contents in green tea were determined by high performance liquid chromatography method, using the mobile phase consisting of acetic acid, methyl alcohol, N,N- dimethylformamide and warter (1:1:40:58, V/V). The column temperature was 30 ℃, and the detection wavelength was 280.0 nm. The regression equation obtained was Y=1×10-7X－0.0036 (r =0.9995). The coefficient of variation was less than 3.04%, and the recovery was 99.16%－100.60%. The result indicates that the proposed method is simple , accurate and feasible for determination of polyphenols in the green teas.

Key words: green tea, HPLC, polyphenols, determination