Extraction Methods of PCR-detectable Escherichia coli Genomic DNA in Milk

doi:10.7506/spkx1002-6630-201024066

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (24): 308-310.doi: 10.7506/spkx1002-6630-201024066

• Analysis & Detection • Previous Articles Next Articles

Extraction Methods of PCR-detectable Escherichia coli Genomic DNA in Milk

SONG Hong-xin1，ZOU Lian-zhu1，MA Dong2，XUE Hai-yan1

(1. College of Life Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, China；
2. North China Coal Medical University, Tangshan 063000, China)

Received:2010-07-26 Revised:2010-11-15 Online:2010-12-25 Published:2010-12-29
Contact: SONG Hong-xin E-mail:hongxinsong@163.com

Abstract

Abstract:

In order to obtain an appropriate sample for PCR detection, four extraction methods including saturated phenol method, CTAB method, kit method and solvent pyrolysis method for E. coli genomic DNA in milk samples were compared. Through evaluating the purity, quantitative analysis and PCR analysis of genomic DNA extracted by four methods, an effective and fast solvent-thermal pyrolysis method was suitable for the extraction of E. coli genomic DNA in milk. The genomic DNA extracted by this method used as the template is suitable for PCR amplification of E. coli DNA, which can be used for the detection of E. coli in milk.

Key words: Dairy products, Escherichia coli, Total genomic DNA, PCR

CLC Number:

TS252.1

SONG Hong-xin1，ZOU Lian-zhu1，MA Dong2，XUE Hai-yan1. Extraction Methods of PCR-detectable Escherichia coli Genomic DNA in Milk[J]. FOOD SCIENCE, 2010, 31(24): 308-310 https://doi.org/10.7506/spkx1002-6630-201024066

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Extraction Methods of PCR-detectable Escherichia coli Genomic DNA in Milk

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