Extraction Methods of PCR-detectable Escherichia coli Genomic DNA in Milk

doi:10.7506/spkx1002-6630-201024066

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (24): 308-310.doi: 10.7506/spkx1002-6630-201024066

• Analysis & Detection • Previous Articles Next Articles

Extraction Methods of PCR-detectable Escherichia coli Genomic DNA in Milk

SONG Hong-xin1，ZOU Lian-zhu1，MA Dong2，XUE Hai-yan1

(1. College of Life Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, China；
2. North China Coal Medical University, Tangshan 063000, China)

Received:2010-07-26 Revised:2010-11-15 Online:2010-12-25 Published:2010-12-29
Contact: SONG Hong-xin E-mail:hongxinsong@163.com

Abstract

Abstract:

In order to obtain an appropriate sample for PCR detection, four extraction methods including saturated phenol method, CTAB method, kit method and solvent pyrolysis method for E. coli genomic DNA in milk samples were compared. Through evaluating the purity, quantitative analysis and PCR analysis of genomic DNA extracted by four methods, an effective and fast solvent-thermal pyrolysis method was suitable for the extraction of E. coli genomic DNA in milk. The genomic DNA extracted by this method used as the template is suitable for PCR amplification of E. coli DNA, which can be used for the detection of E. coli in milk.

Key words: Dairy products, Escherichia coli, Total genomic DNA, PCR

CLC Number:

TS252.1

SONG Hong-xin1，ZOU Lian-zhu1，MA Dong2，XUE Hai-yan1. Extraction Methods of PCR-detectable Escherichia coli Genomic DNA in Milk[J]. FOOD SCIENCE, 2010, 31(24): 308-310.

[1]	GAN Yufei, XUE Zhenglian, ZHOU Jie, WANG Fang, WANG Zhou, LIU Yan. Inhibition and Mechanism of Action of Truncated Phospholiase A1 Accessory Protein S from Serratia marcescens against Escherichia coli [J]. FOOD SCIENCE, 2021, 42(7): 45-51.
[2]	FENG Cuiping, LIU Yinuo, FAN Shuangxi, WU Zhuying, ZHONG Qiding. Determination of Casein Content in Dairy Products by 31P Nuclear Magnetic Resonance [J]. FOOD SCIENCE, 2021, 42(4): 221-226.
[3]	SHAN Shan, HUANG Zhaohong, HUANG Yanmei, LIU Daofeng, LIU Chengwei, HUANG Yunhong, LONG Zhong’er. Rapid Detection of Shiga Toxin-producing Escherichia coli by Immunochromatography Combined with Asymmetric Polymerase Chain Reaction [J]. FOOD SCIENCE, 2021, 42(2): 312-318.
[4]	MEN Jiaxuan, XIONG Bo, HAO Yanan, LI Xuan, LIU Yining, XIE Xixian. Metabolic Engineering of Escherichia coli for Efficient Synthesis of L-Phenylalanine [J]. FOOD SCIENCE, 2021, 42(2): 114-120.
[5]	SUN Yue, LIU Jiayi, CHEN Lu, DU Hong, BAI Fengling, LÜ Xinran, ZHANG Defu, GUO Xiaohua, LI Jianrong. Screening of Lactic Acid Bacteria for Antagonistic Activity against Drug-Resistant Escherichia coli and Underlying Mechanism [J]. FOOD SCIENCE, 2021, 42(2): 121-127.
[6]	ZHAO Linna, LIU Na, WANG Xueshuo, CUI Shenghui. Preparation of Reference Materials for Enteroaggregative Escherichia coli Cells and Genomic DNA [J]. FOOD SCIENCE, 2021, 42(18): 103-110.
[7]	SHAN Shan, HUANG Yanmei, ZHAO Xuelong, CHEN Wenyao, LIU Chengwei, LONG Zhong’er, LIU Daofeng,. Detection of Escherichia coli O157:H7 by Cascade Signal Transduction System Combined with Immunochromatography [J]. FOOD SCIENCE, 2021, 42(18): 314-320.
[8]	XU Xiuli, YAO Guihong, NIE Xuemei, CUI Dongwei, ZHANG Feng. Comparative Analysis of Regulations and Standards for Milk and Dairy Products in China and Abroad with Respect to Main Quality Indices [J]. FOOD SCIENCE, 2021, 42(11): 304-312.
[9]	LI Juan, REN Fang, ZHEN Dawei, ZHANG Hao, XIE Jianchun. Analysis of Volatile Flavor Compounds Dairy Products by Gas Chromatography-Ion Mobility Spectrometry [J]. FOOD SCIENCE, 2021, 42(10): 235-240.
[10]	Fan Yiling, WANG Shujuan, LI Qiongqiong, HU Ying, SONG Minghui, Qin Feng, Liu Hao, YANG Meicheng. Rapid Detection of Shiga Toxin-producing Escherichia coli by Recombinase Polymerase Amplification Combined with Centrifugal Compact Disc Microfluidic Chip [J]. FOOD SCIENCE, 2021, 42(10): 297-304.
[11]	WEI Guangqiang, SHI Yanan, WAN Changjiang, HUANG Aixiang. Characterization of Peptides from Simulated Gastrointestinal Digestion of Ethnic Dairy Products in Yunnan, China [J]. FOOD SCIENCE, 2021, 42(1): 208-214.
[12]	WENG Chen, DENG Zeyuan, LI Jing. Comparison of Lipid Compositions of Several Dairy Products [J]. FOOD SCIENCE, 2020, 41(4): 149-156.
[13]	DU Yan, CHEN Fusheng, CHEN Chen, LIU Ying. Comparative Evaluation of DNA Extraction Methods for Detection of Transgenic Soybean Protein and Analysis of lectin Gene Degradation during Soybean Protein Extraction [J]. FOOD SCIENCE, 2020, 41(4): 102-111.
[14]	WANG Hongyi, LIU Fang, SUN Zhilan, SU Mengmeng, ZHU Yongzhi, WANG Daoying, XU Weimin, PENG Jing. Synergistic Antibacterial Effect and Mechanism of Helveticin-M Combined with Chlorogenic Acid on Escherichia coli and Salmonella enteritidis [J]. FOOD SCIENCE, 2020, 41(3): 68-74.
[15]	WU Lina, DONG Pengcheng, ZHANG Yimin, MAO Yanwei, LIANG Rongrong, YANG Xiaoyin, ZHU Lixian, LUO Xin. Biofilm Formation Ability of Escherichia coli O157:H7 on Stainless Steel Surface and Its Relationship with Bacterial Characteristics [J]. FOOD SCIENCE, 2020, 41(22): 127-132.

Extraction Methods of PCR-detectable Escherichia coli Genomic DNA in Milk

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments