Abstract:

Pepsin hydrolysis was used to extract and prepare collagen from deer tendons. The optimal extraction conditions were explored by single factor experiments and orthogonal experiments to be acetic acid concentration of 5.5%, material-to-liquid ratio of 1:9, extraction time of 10 h, pepsin-substrate ratio of 1:1000, and hydrolysis time of 24 h. The yield of deer tendon collagen was 74.56% and protein content was 65.54%. The optimized extraction method for deer tendon collagen was characteristic of simple operation and high extraction rate.

Key words: deer tendon, collagen, pepsin, HPGFC