Loop-mediated Isothermal Amplification Assay for the Detection of Salmonella in Meat and Meat Products

doi:10.7506/spkx1002-6630-201016059

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (16): 270-273.doi: 10.7506/spkx1002-6630-201016059

• Analysis & Detection • Previous Articles Next Articles

Loop-mediated Isothermal Amplification Assay for the Detection of Salmonella in Meat and Meat Products

WANG Yu，WANG Zhen-qiang，ZHANG Wei*

(College of Food Science and Technology, Agricultural University of Hebei, Baoding 071000, China)

Received:2010-06-22 Online:2010-08-25 Published:2010-12-29
Contact: ZHANG Wei* E-mail:zhangwei631126@163.com

Abstract

Abstract:

A novel nucleic acid amplification method, termed loop-mediated isothermal amplification (LAMP), which amplifies DNA with high specificity, efficiency, and rapidity under isothermal conditions, may be a valuable tool for the rapid detection of infectious diseases. In this study, two pairs of gene-specific primers for the conservative invA gene were designed to establish a LAMP assay for detecting Salmonella. The assay method was applied to detect meat of different animal species, and proved highly sensitive and specific and time saving.

Key words: loop-mediated isothermal amplification, detection, Salmonella, meat and meat products

CLC Number:

Q939.1
Q789

WANG Yu，WANG Zhen-qiang，ZHANG Wei*. Loop-mediated Isothermal Amplification Assay for the Detection of Salmonella in Meat and Meat Products[J]. FOOD SCIENCE, 2010, 31(16): 270-273.

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Loop-mediated Isothermal Amplification Assay for the Detection of Salmonella in Meat and Meat Products

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