Using Western Blotting to Identify Major Allergens of Four Species of Shrimp

doi:10.7506/spkx1002-6630-201016060

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (16): 274-277.doi: 10.7506/spkx1002-6630-201016060

• Analysis & Detection • Previous Articles Next Articles

Using Western Blotting to Identify Major Allergens of Four Species of Shrimp

WU Hai-ming，HU Zhi-he*，WANG Chao

Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science,
Tianjin University of Commerce, Tianjin 300134, China

Received:2010-01-21 Online:2010-08-25 Published:2010-12-29
Contact: HU Zhi-he*， E-mail:hzhihe@tjcu.edu.cn

Abstract

Abstract:

Litopenaeus vannamei, Fenneropenaeus chinensis, Metapenaeus ensis and mantis shrimp were evaluated for their major allergens. Composition analysis of protein extracts from these four species of shrimp was carried out using SDS-PAGE, and western blotting was then used to identify their major allergens. The major allergens of Litopenaeus vannamei were protein fractions of 99, 33, 19 kD and 14 kD in molecular weight, and those of Fenneropenaeus chinensis protein fractions of 39, 33 kD and 28 kD, and those of Metapenaeus ensis protein fractions of 33 kD and 24 kD, and those of mantis shrimp protein fractions of 56 kD and 48 kD.

Key words: shrimp, allergen, western blotting

CLC Number:

WU Hai-ming，HU Zhi-he*，WANG Chao. Using Western Blotting to Identify Major Allergens of Four Species of Shrimp[J]. FOOD SCIENCE, 2010, 31(16): 274-277.

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Using Western Blotting to Identify Major Allergens of Four Species of Shrimp

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