Abstract:

The purpose of the present study was to evaluate the use of macroporous resin adsorption for purifying crude valtrate extract from Valeriana officinalis L.var. latifolia Miq. Five macroporous resins were tested for their static adsorption/desorption performance towards Valeriana officinalis L.var. latifolia Miq valtrate, and D101 resin was the selected adsorbent. The optimal values of sample concentration and sample loading amount were determined to be 10.0 mg/mL and 20 mL/13 g resin, respectively, and an amount of desorption solvent (aqueous methanol solution) of 4 BV was found enough to recover adsorbed valtrate. A quadratic general rotary unitized design involving 20 experiments of 3 variables at 5 levels combined with multiple regression analysis leading to a quadratic regression model was employed to deal with the effects of sample flow rate, desorption flow rate and methanol concentration in aqueous solution on valtrate recovery. The optimal values of the process conditions were found to be: sample flow rate 2.5 BV/h, desorption flow rate 1.7 BV/h and methanol concentration in aqueous solution 75%. Under the optimized conditions, the theoretical and experimental values of valtrate recovery from crude extract from Valeriana officinalis L.var. latifolia Miq were 72.40% and (72.12± 0.1)%, respectively.

Key words: Valeriana officinalis L.var. latifolia Miq, valtrate, purification, macroporous resin