Optimization of Enymolysis Conditions for Preparation of Pleurotus eryngii Protoplasts

doi:10.7506/spkx1002-6630-201010031

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (10): 155-158.doi: 10.7506/spkx1002-6630-201010031

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Optimization of Enymolysis Conditions for Preparation of Pleurotus eryngii Protoplasts

LI Yan-li1，JIN Zhou-yu1，LI Yu2,*

1. College of Life Science, Jilin Agricultural University, Changchun 130118, China；
2. Engineer Research Center of Edible and Medicinal Fungi, Ministry of Education, Changchun 130118, China

Received:2009-08-10 Online:2010-05-15 Published:2010-12-29
Contact: LI Yu E-mail:yuli966@126.com

Abstract

Abstract:

The optimal conditions for the lywallzyme hydrolysis of the cultured mycelia of Pleurotus eryngii for protoplast production were investigated by single factor and orthogonal array design methods. Results showed that the optimal values of hydrolysis conditions were as follows: substrate concentration 133 mg/mL, enzyme dosage 1.0% and pH 6.0 for a hydrolysis duration of 2.5 h at 30 ℃. The hydrolysate obtained under such conditions exhibited a protoplast density of up to 6.34 × 107 cells/mL.

Key words: lywallzyme, Pleurotus eryngii, protoplasts, enzymolysis conditions, orthogonal array design

CLC Number:

S646

LI Yan-li1，JIN Zhou-yu1，LI Yu2,*. Optimization of Enymolysis Conditions for Preparation of Pleurotus eryngii Protoplasts[J]. FOOD SCIENCE, 2010, 31(10): 155-158.

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Optimization of Enymolysis Conditions for Preparation of Pleurotus eryngii Protoplasts

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