Optimization of Enymolysis Conditions for Preparation of Pleurotus eryngii Protoplasts

doi:10.7506/spkx1002-6630-201010031

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (10): 155-158.doi: 10.7506/spkx1002-6630-201010031

• Processing Technology • Previous Articles Next Articles

Optimization of Enymolysis Conditions for Preparation of Pleurotus eryngii Protoplasts

LI Yan-li1，JIN Zhou-yu1，LI Yu2,*

1. College of Life Science, Jilin Agricultural University, Changchun 130118, China；
2. Engineer Research Center of Edible and Medicinal Fungi, Ministry of Education, Changchun 130118, China

Received:2009-08-10 Online:2010-05-15 Published:2010-12-29
Contact: LI Yu E-mail:yuli966@126.com

Abstract

Abstract:

The optimal conditions for the lywallzyme hydrolysis of the cultured mycelia of Pleurotus eryngii for protoplast production were investigated by single factor and orthogonal array design methods. Results showed that the optimal values of hydrolysis conditions were as follows: substrate concentration 133 mg/mL, enzyme dosage 1.0% and pH 6.0 for a hydrolysis duration of 2.5 h at 30 ℃. The hydrolysate obtained under such conditions exhibited a protoplast density of up to 6.34 × 107 cells/mL.

Key words: lywallzyme, Pleurotus eryngii, protoplasts, enzymolysis conditions, orthogonal array design

CLC Number:

S646

LI Yan-li1，JIN Zhou-yu1，LI Yu2,*. Optimization of Enymolysis Conditions for Preparation of Pleurotus eryngii Protoplasts[J]. FOOD SCIENCE, 2010, 31(10): 155-158 https://doi.org/10.7506/spkx1002-6630-201010031

[1]	TIAN Jiangrong, ZHONG Lei, JI Yang, HU Qiuhui, ZHAO Liyan. Isolation, Purification, Structural Characterization and Immunoregulatory Activity of Pleurotus eryngii Peptides [J]. FOOD SCIENCE, 2025, 46(11): 94-104.
[2]	SONG Ling, LU Juan, LIU Yurong, LUO Ruiping, ZHOU Xing, GUI Mingying. Differences in the Flavor Pleurotus eryngii at Different Growth Stages under Two Crop Rotation Systems [J]. FOOD SCIENCE, 2025, 46(10): 207-215.
[3]	ZHU Kai, WU Weijie, FANG Xiangjun, CHEN Hangjun, LIU Ruiling, HAN Yanchao, GAO Haiyan. Effect of Light Emitting Diode Blue Light Combined with Ultraviolet Treatment on the Storage Quality of Fresh-Cut Pleurotus eryngii [J]. FOOD SCIENCE, 2022, 43(9): 223-231.
[4]	MA Gaoxing, WANG Han, YANG Wenjian, SU Anxiang, PEI Fei, MA Ning, HU Qiuhui. Effects of Different Extraction Processes on Structural Characteristic and Immunomodulatory Activity of Pleurotus eryngii Polysaccharide [J]. FOOD SCIENCE, 2022, 43(17): 42-49.
[5]	ZHANG Xueli, HU Qiuhui, JI Yang, YU Anqi, ZHONG Lei, ZHAO Liyan, FAN Yuming. Nutritional and Structural Characteristics of Selenium-Enriched Protein from Pleurotus eryngii and Its Alleviative Effect on Lead Toxicity [J]. FOOD SCIENCE, 2022, 43(15): 125-133.
[6]	PENG Dan, LIU Yali, LI Linqing, BI Yanlan. Optimization of Modeling Conditions for Near Infrared Measurement of Protein Content in Milk by Orthogonal Array Design [J]. FOOD SCIENCE, 2020, 41(4): 256-261.
[7]	HUANG Ziyu, LIN Yingying, SONG Sijia, REN Fazheng, GUO Huiyuan,. Optimization of Cleaning-in-place Regime for Removal of Bacillus cereus Biofilm [J]. FOOD SCIENCE, 2020, 41(14): 138-145.
[8]	LI Qiaozhen, LI Xiaobei, WU Di, LI Zhengpeng, LI Yu, ZHOU Feng, YANG Yan. Analysis and Evaluation of Volatile Compounds in Different Strains of Industrially Cultivated Pleurotus eryngii [J]. FOOD SCIENCE, 2019, 40(6): 266-271.
[9]	ZHENG Hengguang, SHEN Hengsheng, YANG Daofu, WENG Minjie, CHEN Junchen. Structural Characterization and Antitumor Activity of Crude Polysaccharide Extracted from the Stalk Residue of Pleurotus eryngii [J]. FOOD SCIENCE, 2019, 40(22): 7-13.
[10]	WU Yiliang, ZHONG Lei, MA Ning, PEI Fei, MA Gaoxing, HU Qiuhui. Immunoregulatory Effect of Soybean Protein Isolate-Pleurotus eryngii Polysaccharide Conjugate on RAW264.7 Cells [J]. FOOD SCIENCE, 2019, 40(17): 202-207.
[11]	MA Qi, BO Jifang, FENG Li, NAI Yifang, WANG Xiaojing, LI Mei*, XU Huaide. Effect of Drying Method on Volatile Components of Pleurotus eryngii Analyzed by Combined Use of GC-MS and Electronic Nose [J]. FOOD SCIENCE, 2019, 40(14): 276-282.
[12]	ZHANG Pengyun, LI Rong, CHEN Lisi, LIN Shumian, YANG Fang, ZHANG Feng. Analysis of Volatile Compounds in Pueraria Radix Using Headspace Solid-Phase Microextraction-Gas Chromatography-Mass Spectrometry (HS-SPME-GC-MS) Combined with Automated Mass Spectral Deconvolution and Identification System (AMDIS) [J]. FOOD SCIENCE, 2019, 40(12): 220-225.
[13]	LI Chunhong, ZHANG Leigang, LUO Shufen, ZHOU Hongsheng, HU Huali, LI Pengxia. Effect of Exogenous Ethylene Treatment on Endogenous Ethylene Production and Senescence of Postharvest Pleurotus eryngii [J]. FOOD SCIENCE, 2018, 39(23): 182-191.
[14]	LIU Tingting, LIU Yang, ZHANG Jing, SONG Yunyu, LI Guijie, ZHANG Yanrong. Optimization of Extraction and Characterization of Protein from Allium cepa L. var. agrogatum Don [J]. FOOD SCIENCE, 2018, 39(2): 267-272.
[15]	WEI Junhui, XUE Yuan, FENG Li, ZHANG Ruoxi, WANG Xiaojing, LEI Hongjie, XU Huaide. Physicochemical and Functional Properties of Pleurotus eryngii Protein Isolate and Albumin [J]. FOOD SCIENCE, 2018, 39(18): 54-60.

Optimization of Enymolysis Conditions for Preparation of Pleurotus eryngii Protoplasts

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments