Application of Nano-biosensor to Chloramphenicol Detection

doi:10.7506/spkx1002-6300-201008036

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (8): 167-170.doi: 10.7506/spkx1002-6300-201008036

Application of Nano-biosensor to Chloramphenicol Detection

WU Hui-juan1，WEI Ling1，LIU Qing-jun1,*，YUE Jia-chang2，LI Bao-ming1，ZHANG Xiao-li1，GAO Li-juan1，KANG Zi-jia1，ZHAO Long1，QIAN Jia-lin1

1. Beijing Centre for Physical and Chemical Analysis, Beijing 100094, China；
2. Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

Received:2009-04-16 Online:2010-04-15 Published:2010-12-29
Contact: LIU Qing-jun1 E-mail:Liuqj@bjast.ac.cn

Abstract

Abstract:

Chloramphenicol is a broad-spectrum antibiotic and its use is prohibited in many countries in the world due to its toxicity and side effects. The detection of chloramphenicol residue in food products has become a critical technical barrier. F1300, a fluorescence indicator, makes it easy to quantify the activity of F0F1-ATPase. Based on this, a complex of chloramphenicol antibody-biotin-streptavidin-biotin- β subunit antibody was constructed and connected to β subunits of F0F1-ATPase in the chromatophore. The concentration of chloramphenicol was measured according to the relative fluoresent value of F0F1-ATPase biosensor tagged by F1300. The detection limit of this method was 1 × 10-11 mg/mL. Only 35 min was needed for the whole detection process. Therefore, this method, thanks to its rapidity, simplicity and high sensitivity, will has a promising application to chloramphenicol detection.

Key words: nano-biosensor, chloramphenicol, detection

CLC Number:

TS207.53

WU Hui-juan1，WEI Ling1，LIU Qing-jun1,*，YUE Jia-chang2，LI Bao-ming1，ZHANG Xiao-li1，GAO Li-juan1，KANG Zi-jia1，ZHAO Long1，QIAN Jia-lin1. Application of Nano-biosensor to Chloramphenicol Detection[J]. FOOD SCIENCE, 2010, 31(8): 167-170.

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Application of Nano-biosensor to Chloramphenicol Detection

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