FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (7): 190-195.doi: 10.7506/spkx1002-6300-201007042

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Screening of RAPD Markers Linked to Acid Resistance-related Gene in Oenococcus oeni

LIU Shu-wen1,2,LI Xi-zhu2,WANG Zuo-jing2,ZHOU Li-guo2   

  1. 1. College of Enology, Northwest A&F University, Yangling 712100, China;
    2. Shaanxi Engineering Research Center for Viti-Viniculture, Yangling 712100, China
  • Received:2009-08-27 Online:2010-04-01 Published:2010-12-29
  • Contact: LIU Shu-wen E-mail:liushuwen@nwsuaf.edu.cn

Abstract:

Major factors affecting RAPD reaction system were optimized and an optimal RAPD reaction system was established for laboratory investigations. Subsequently, 33 acid-resistant Oenococcus oeni strains and 9 acid-sensitive Oenococcus oeni strains were used as experimental materials for screening RAPD markers linked to acid resistance-related genes. Random amplified polymorphic DNA (RAPD) and bulked segregate analysis (BSA) techniques were employed in this study. Three RAPD markers S40-1400, S333-2500 and S333-650 linked to acid resistance in Oenococcus oeni were obtained through a selection from 45 random primers and confirmation in a single Oenococcus oeni strain.

Key words: Oenococcus oeni, acid-resistant gene, RAPD markers

CLC Number: