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Sequence-characterized Amplified Region (SCAR) Marker Linked to Acid Resistance-related Gene in Lactobacillus plantarum

LI Kai1,ZHU Cheng-long1,LÜ Zhen1,YANG Rui1,DAN Xia1,LIU Shu-wen1,2,*   

  1. 1. College of Enology, Northwest A&F University, Yangling 712100, China;
    2. Shaanxi Engineering Research Center for Viti-viniculture, Yangling 712100, China
  • Online:2013-10-15 Published:2013-09-27
  • Contact: LIU Shu-wen

Abstract:

Totally 11 acid-resistant Lactobacillus plantarum strains and 8 acid-sensitive Lactobacillus plantarum strains
were investigated, and random amplified polymorphic DNA (RAPD) and bulked segregate analysis (BSA) techniques were
employed in this study. One RAPD marker S116-680 related to acid resistance in Lactobacillus plantarum was obtained
through the selection from 55 random primers and the confirmation in a single Lactobacillus plantarum strain. The specific
fragments were recovered and ligated into pGEM-T Easy vector, and then sequenced. According to the sequence information,
a pair of specific primers (SA1/SA2) were designed, and SA1/SA2 with stable amplification of a 680bp specific band from
11 acid-resistant strains, was named as SA-680 acid resistance-related marker.

Key words: Lactobacillus plantarum, acid-resistant gene, RAPD marker, SCAR marker

CLC Number: