Pigment Composition and Anticancer Effect on Hepatoma Carcinoma of Greening Garlic
LIU Wei, WANG Jingya, CHEN Haixia
2018, 39(21):
135-141.
doi:10.7506/spkx1002-6630-201821020
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Objective: To investigate the inhibitory effect of two pigments extracted from greening garlic on hepatoma carcinoma. Methods: Green garlic was extracted with 75% cold ethanol and the crude extract was separated by AB-8 macroporous resin column chromatography to obtain blue pigment (BP) and yellow pigment (YP). The 3-(4,5-dimethyl-2- thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide method was used to investigate the inhibitory effect of purified BP-1 and YP-3 on the proliferation of HepG2 cells. An H22 tumor-bearing mouse model was established and 80 H22 tumor-bearing mice were divided into 8 groups: model group, positive control group (cyclophosphamide, CTX, 20 mg/kg), BP low-, middle- and high-dose groups (L-BP, M-BP, H-BP; 75, 150, 300 mg/kg), and YP low-, middle- and high-dose groups (L-YP, M-YP, H-YP; 75, 150, 300 mg/kg) for 14 days, with the aim to study the effects of the pigments on tumor growth, immune organ index, and water and food efficiency. The activities of superoxide dismutase, glutathione peroxidase, catalase, alanine aminotransferase, aspartate aminotransferase and malondialdehyde (MDA) content in liver and the levels of cytokines tumor necrosis factor alpha (TNF-α), interleukin-2 (IL-2) and vascular endothelial growth factor (VEGF) in serum were measured. Pathological analysis of tumor tissues in mice was carried out. Results: Compared with the model group, the tumor volume in the BP and YP groups were significantly smaller (P < 0.05) accompanied by a slower growth rate of transplanted tumors, and the thymus and spleen indexes were significantly increased (P < 0.05), suggesting improved immune function and inhibited tumor growth in H22 tumor-bearing mice. Moreover, both pigments increased the activity of antioxidant enzymes, decreased the content of MDA, and consequently attenuated liver inflammation. Compared with the model group, the expression levels of IL-2 and TNF-α in the treatment groups were increased (P < 0.05), and the expression level of VEGF was decreased by 63%. Conclusion: BP and YP from green garlic have anticancer activity on hepatoma carcinoma, which lays the foundation for the development of garlic-based functional foods.