小鼠血浆中槲皮素脂溶性及水溶性代谢产物的分离与测定

doi:10.7506/spkx1002-6630-201206029

食品科学 ›› 2012, Vol. 33 ›› Issue (6): 132-136.doi: 10.7506/spkx1002-6630-201206029

小鼠血浆中槲皮素脂溶性及水溶性代谢产物的分离与测定

许春莲，胡晓鹃，邱奇琦，申明月，舒翔，聂少平*，谢明勇

南昌大学食品科学与技术国家重点实验室

出版日期:2012-03-25 发布日期:2012-03-03
基金资助:
科技部国际合作项目(2010DFA31780)

Separation and Determination of Lipid-Soluble and Water-Soluble Metabolites of Qucertin in Mouse Plasma

XU Chun-lian，HU Xiao-juan，QIU Qi-qi，SHEN Ming-yue，SHU Xiang，NIE Shao-ping*，XIE Ming-yong

(State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China)

Online:2012-03-25 Published:2012-03-03

摘要/Abstract

摘要： 目的：分离和测定小鼠口服槲皮素单体后血浆中的槲皮素脂溶性和水溶性代谢产物。方法：血浆经正己烷-氯仿(1:1，V/V)、甲醇洗脱分离槲皮素脂溶性和水溶性代谢产物，正己烷洗脱部分经胰脂肪酶酶解，甲醇洗脱部分经硫酸酯酶、β-葡萄糖醛酸酶水解，以体积分数0.1%甲酸溶液-乙腈为流动相梯度洗脱，使用四极杆-飞行时间串联质谱仪检测，负离子条件下进行扫描，分析在单次给予昆明小鼠槲皮素单体后血浆中槲皮素脂溶、水溶代谢产物情况。结果：槲皮素在血液中脂溶性代谢产物占84.38%，水溶性代谢产物较少，槲皮素与脂肪酸成酯在槲皮素代谢中占主要部分。结论：该方法准确性高、重现性好，可有效分离和检测血液中槲皮素脂溶性和水溶性代谢产物。

关键词: 槲皮素, 四极杆-飞行时间串联质谱仪, 脂溶性, 代谢产物

Abstract: Objective: To separate and determine lipid-soluble and water-soluble metabolites of qucertin in the plasma of Kunming mice. Methods: Three week-old Kunming mice were administrated with quercetin at a dose of 100 mg/kg by gavage. Lipid-soluble and water-soluble metabolites of qucertin in mouse plasma were separated by elution with n-hexane/chloroform (1:1) and methanol, respectively. Then, the n-hexane/chloroform eluate was hydrolyzed by pancreatic lipase, and the methanol eluate simultaneously by sulfatase and β-glucuronidase. Lipid-soluble and water-soluble metabolites of qucertin in the plasma of Kunming mice were analyzed by tandem quadrupole-time of flight mass spectrometry in a negative ion mode based on gradient elution with a mobile phase composed of 0.1% formic acid (A) and acetonitrile (B). Results: In mouse plasma 84.38% of quercetin was metabolized to form esters, while only a small amount of water-soluble metabolites were found. Quercetin esters were the predominant metabolites in mouse plasma. Conclusions: The method is highly accurate and reproducible and can allow effective separation and determination of lipid-soluble and water-soluble metabolites of qucertin in plasma.

Key words: quercetin, quadrupole-time of flight (Q-TOF), lipid-soluble, metabolites

中图分类号:

许春莲，胡晓鹃，邱奇琦，申明月，舒翔，聂少平，谢明勇. 小鼠血浆中槲皮素脂溶性及水溶性代谢产物的分离与测定[J]. 食品科学, 2012, 33(6): 132-136.

XUChun-lian，HUXiao-juan，QIUQi-qi，SHENMing-yue，SHUXiang，NIEShao-ping，XIEMing-yong. Separation and Determination of Lipid-Soluble and Water-Soluble Metabolites of Qucertin in Mouse Plasma[J]. FOOD SCIENCE, 2012, 33(6): 132-136.

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小鼠血浆中槲皮素脂溶性及水溶性代谢产物的分离与测定

Separation and Determination of Lipid-Soluble and Water-Soluble Metabolites of Qucertin in Mouse Plasma

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