• 食品化学 •

### 荞麦蜂花粉破壁扫描电子显微镜分析及黄酮模拟消化释放

1. （云南民族大学化学与环境学院，云南省跨境民族地区生物质资源清洁利用国际联合研究中心，生物基材料绿色制备技术国家地方联合工程研究中心，云南 昆明 650500）
• 出版日期:2020-06-25 发布日期:2020-06-22
• 基金资助:
国家自然科学基金地区科学基金项目（21367025）

### Scanning Electron Microscope Analysis of Cell Wall Breakdown of Buckwheat Bee Pollen and Release of Flavonoids during Its Simulated Gastrointestinal Digestion

SHEN Zhiyan, GAO Yuntao, ZHANG Haifen, JIANG Qiongfang, XIONG Huabin

1. (Joint Research Centre for International Cross-border Ethnic Regions Biomass Clean Utilization in Yunnan, National and Local Joint Engineering Research Center for Green Preparation Technology of Biobased Materials, College of Chemistry and Environment, Yunnan Minzu University, Kunming 650500, China)
• Online:2020-06-25 Published:2020-06-22

Abstract: Low-temperature energy-focusing ultrasound was used to break down the cell wall of buckwheat bee pollen and the process was analyzed using scanning electron microscope (SEM). In the microscopic field, the bee pollen cells were spited, ruptured along the germ ditch, and broken into pieces successively, which allows the establishment of an accurate method for calculating the efficiency of cell wall disruption. Based on SEM observation, the optimum experimental conditions that provided the maximum efficiency of cell wall disruption (98.33%) were determined as follows: initial temperature 0 ℃, ultrasound power 400 W, pulse duty factor 1:2, sonication time 30 min, and ratio of solid to solvent 1:30 (g/mL). Further study showed that the efficiency of cell wall disruption displayed a linear relationship with the release percentage of total flavonoids from the pollen when incubated in simulated gastric and intestinal fluid, which was fitted into the following equation was y = 0.363 8x + 27.172 with a correlation coefficient (R2) of 0.999 7.