关键词: 苯并咪唑, 固相萃取, 凝胶渗透色谱, 液相色谱串联质谱

Abstract:

The eel samples were purified by solid phase extraction (SPE) and gel permeation chromatography (GPC) respectively, and then benzimidazole anthelmintics residues in them were determined by liquid chromatography and tandem mass spectrometry (LC-MS-MS) method. Therefore, two detection methods with two different pre-treatments were developed and compared for the quantitative determination of residues of six benzimidazole anthelmintics including mebendazole (MEB), albendazole (ALB), oxibendazole (OXI), oxfendazole (OXF), fenbendazole (FEN) and flubendazole (FLU). The results of eight repeated tests for three levels (2.0, 4.0 and 8.0  μg/kg) of six benzimidazole anthelmintics showed that both of detection limits of two methods are below 2.0  μg/kg, and both of recoveries are between 70% and 110% with a RSD of less than 15%. All of these indexes accord to the requirements of the residue detection.

Key words: benzimidazole, solid phase extraction (SPE), gel permeation chromatography (GPC), liquid chromatography and tandem mass spectrometry (LC-MS-MS)