在线固相萃取-液相色谱-串联质谱法测定鸡肉和鸡蛋中5 种抗病毒类药物残留量

doi:10.7506/spkx1002-6630-20200820-269

食品科学 ›› 2021, Vol. 42 ›› Issue (10): 305-310.doi: 10.7506/spkx1002-6630-20200820-269

在线固相萃取-液相色谱-串联质谱法测定鸡肉和鸡蛋中5 种抗病毒类药物残留量

马俊美，何亮娜，孙磊，王东，张雷雷，张岩，康文艺

（1.河北省法医学重点实验室，河北医科大学法医学院，河北石家庄 050017；2.河北省食品检验研究院，河北省食品安全重点实验室，河北石家庄 050227；3.河南大学国家食用菌加工技术研发专业中心，河南开封 475004）

出版日期:2021-05-25 发布日期:2021-06-02
基金资助:
“十三五”国家重点研发计划重点专项（2018YFC1603400）；河北省高层次人才资助项目（A2017005008）

Determination of Five Antiviral Drug Residues in Chicken Meat and Eggs Using On-line Solid Phase Extraction Coupled to Liquid Chromatography-Tandem Mass Spectrometry

MA Junmei, HE Liangna, SUN Lei, WANG Dong, ZHANG Leilei, ZHANG Yan, KANG Wenyi

(1. Hebei Key Laboratory of Forensic Medicine, College of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China;2. Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050227, China;3. National R&D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China)

Online:2021-05-25 Published:2021-06-02

摘要/Abstract

摘要： 建立鸡肉和鸡蛋中阿比多尔、金刚烷胺、金刚乙胺、美金刚、奥司他韦的在线固相萃取-液相色谱-串联质谱检测方法。样品采用1.0%甲酸-乙腈提取，经MCX在线固相萃取小柱净化，用XBridge C18色谱柱分离，使用电喷雾离子源正离子模式监测，以内标法定量。结果表明：5 种抗病毒类药物在0.05～100 ng/mL范围内线性关系良好；检出限为0.05～0.10 μg/kg，定量限为0.10～0.20 μg/kg；方法回收率范围为80.03%～93.96%，相对标准偏差低于10%。该方法分析速度快、灵敏度高，可用于鸡肉和鸡蛋中5 种抗病毒类药物的定性、定量分析。

关键词: 在线固相萃取；液相色谱-串联质谱法；抗病毒类药物；内标法定量

Abstract: In this research, we developed an on-line solid phase extraction (SPE) combined with liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the detection of arbidol, amantadine, rimantadine, memantine and oseltamivir in chicken meat and eggs. Samples were extracted with 1.0% formic acid in acetonitrile, and the extract was purified using an MCX on-line solid phase extraction cartridge. The target substances were separated using an XBridge C18 column, detected by electrospray ionization in the positive ion mode, and quantitated by the internal standard method. The results showed that the calibration curves of all analytes had a good linear relationship in the concentration range of 0.05–100 ng/mL. The limit of detection (LOD) and the limit of quantitation (LOQ) were 0.05–0.10 μg/kg and 0.10–0.20 μg/kg, respectively. The recoveries of the developed method were in the range of 80.03%–93.96%, with relative standard deviations (RSDs) of lower than 10%. The method was proven to be simple and sensitive, and could be used for the qualification and quantification of the five antiviral drugs in chicken meat and eggs.

Key words: on-line solid phase extraction; liquid chromatography-tandem mass spectrometry; antiviral drugs; internal standard quantification

中图分类号:

O657.63

马俊美，何亮娜，孙磊，王东，张雷雷，张岩，康文艺. 在线固相萃取-液相色谱-串联质谱法测定鸡肉和鸡蛋中5 种抗病毒类药物残留量[J]. 食品科学, 2021, 42(10): 305-310.

MA Junmei, HE Liangna, SUN Lei, WANG Dong, ZHANG Leilei, ZHANG Yan, KANG Wenyi. Determination of Five Antiviral Drug Residues in Chicken Meat and Eggs Using On-line Solid Phase Extraction Coupled to Liquid Chromatography-Tandem Mass Spectrometry[J]. FOOD SCIENCE, 2021, 42(10): 305-310.

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在线固相萃取-液相色谱-串联质谱法测定鸡肉和鸡蛋中5 种抗病毒类药物残留量

Determination of Five Antiviral Drug Residues in Chicken Meat and Eggs Using On-line Solid Phase Extraction Coupled to Liquid Chromatography-Tandem Mass Spectrometry

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