食品科学 ›› 2009, Vol. 30 ›› Issue (3): 180-183.doi: 10.7506/spkx1002-6630-200903040

• 生物工程 • 上一篇    下一篇

四种大麦间泡沫蛋白质含量差异的比较

孙 俊1,徐 凯2,孙丽华2,张天雪2,赵长新2 ,*   

  1. 1. 辽宁出入境检验检疫局
    2. 大连工业大学生物与食品工程学院
  • 收稿日期:2008-03-05 修回日期:2008-10-29 出版日期:2009-02-01 发布日期:2010-12-29
  • 通讯作者: 赵长新2 ,* E-mail:zhanochangxing@126.com
  • 基金资助:

    辽宁省教育厅发酵工程重点实验室开放课题项目(2004LN-FJ-004)

Analysis and Quantification of Foam-active Protein in Four Cultivars of Barley and Malt

SUN Jun1,XU Kai2,SUN Li-hua2,ZHANG Tian-xue2,ZHAO Chang-xin2,*   

  1. (1. Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian 116001, China ;
    2. School of Biology and Food Engineering, Dalian Polytechnic University, Dalian 116034, China)
  • Received:2008-03-05 Revised:2008-10-29 Online:2009-02-01 Published:2010-12-29
  • Contact: ZHAO Chang-xin2,* E-mail:zhanochangxing@126.com

摘要:

本实验建立了从麦芽中提取泡沫蛋白的便捷方法,并利用考马斯亮蓝法(Bradford 法)和SDS-PAGE 电泳技术进行定量分析,直观地检测和比较了四种大麦和麦芽中泡沫蛋白质的含量、组分和各组分的相对含量及其变化情况,可为评估大麦和麦芽质量以及预测啤酒泡沫的品质提供借鉴。

关键词: 大麦, 泡沫蛋白, 啤酒品质

Abstract:

A rapid and simple method was developed for extracting malt-derived foam-active protein, and the foam-active protein was analyzed and quantified by means of Bradford method and SDS-PAGE. Four barley cultivars grown in different geographic regions were analyzed by these procedures. Based on the analysis results, the qualities of malt and beer foam were predicted. The prediction results accord with actual situation.

Key words: barley (Hordeum vulgare L), foam-active protein, beer quality

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