青蛇果原花青素分离和低聚体纯品的制备

doi:10.7506/spkx1002-6630-200917026

食品科学 ›› 2009, Vol. 30 ›› Issue (17 ): 113-119.doi: 10.7506/spkx1002-6630-200917026

青蛇果原花青素分离和低聚体纯品的制备

肖俊松¹，曹雁平¹，龚玉石²，谢笔钧^{2 ,*}

1. 北京工商大学化学与环境工程学院，北京 100037；2. 华中农业大学食品科学技术学院，湖北武汉 430070

收稿日期:2009-06-22 出版日期:2009-09-01 发布日期:2014-04-14
通讯作者: 谢笔钧 E-mail:bijunxie@sina.com
基金资助:
国家自然科学基金项目(30270938)；“十一五”国家科技支撑计划项目(2006BAD27B08)

Fractionation of Procyanidins from Granny Smith Apples According to Degree of Polymerization and Preparation of Pure Procyanidin Oligomers

XIAO Jun-song1，CAO Yan-ping1，GONG Yu-shi2，XIE Bi-jun2,*

1. School of Chemical and Enviromental Engineering, Beijing Technology and Business University, Beijing 100037, China；
2. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Received:2009-06-22 Online:2009-09-01 Published:2014-04-14
Contact: XIE Bi-jun E-mail:bijunxie@sina.com

摘要/Abstract

摘要：

青蛇果中含有丰富的原花青素，从青蛇果中提取和制备原花青素低聚体，对于原花青素结构和活性的研究具有重要的意义。青蛇果中的原花青素经过水浸提，ADS-17 大孔树脂纯化，TSK HW-40s 树脂层析分级后，经过高效液相- 电喷雾离子化质谱联用(LC-ESI-MS)分析，再通过圆二色谱(CD)和核磁共振(NMR)进行鉴定。青蛇果原花青素最终按聚合度分成了9 个组分，分别含有槲皮素和根皮素及其糖苷，儿茶素和表儿茶素，二聚体、三聚体、四聚体、五聚体等。选择性的收集级份，可以得到纯度较高的原花青素二聚体B2(epicatechin-(4β-8)-epicatechin)和三聚体C1(epicatechin-(4β-8)- epicatechin-(4β-8)-epicatechin)。

关键词: 青蛇果, 原花青素, 低聚体, LC-ESI-MS

Abstract:

Procyanidins are a class of condensed tannins widely present in natural plants with various bio-activities, which are closely related to their degree of polymerization. The preparation of oligomeric procyanidins from Granny Smith apple, which are believed to contain ample procyanidins, will facilitate the study of procyanidins structures and bio-activities. Procyanidins in Granny Smith apples were extracted with water, purified by ADS-17 macroporous resin and fractionated by TSK HW-40s resin and 9 fractions were obtained. High performance liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) analysis indicated that these fractions were composed of quercetin and phloridzin, catechin and epicatechin, and dimers, trimers, tetramers and pentamers in. Procyanidins B2 (epicatechin-(4β-8)-epicatechin) and C1 (epicatechin-(4β-8)-epicatechin-(4β-8)-epicatechin) with relatively high purity were prepared by slective collection of fractions, and were further identified by circular dichroism (CD) and nuclear magnetic resonance (NMR).

Key words: Granny Smith apple, procyanidins, oligomer, LC-ESI-MS

中图分类号:

TS255.36

肖俊松1，曹雁平1，龚玉石2，谢笔钧2,*. 青蛇果原花青素分离和低聚体纯品的制备[J]. 食品科学, 2009, 30(17 ): 113-119.

XIAO Jun-song1，CAO Yan-ping1，GONG Yu-shi2，XIE Bi-jun2,*. Fractionation of Procyanidins from Granny Smith Apples According to Degree of Polymerization and Preparation of Pure Procyanidin Oligomers[J]. FOOD SCIENCE, 2009, 30(17 ): 113-119.

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青蛇果原花青素分离和低聚体纯品的制备

Fractionation of Procyanidins from Granny Smith Apples According to Degree of Polymerization and Preparation of Pure Procyanidin Oligomers

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