食品科学 ›› 2008, Vol. 29 ›› Issue (5): 145-148.

• 基础研究 • 上一篇    下一篇

不同因素对酪蛋白酶解产物螯合亚铁盐能力的影响

 毛学英, 李明, 李敏, 徐世平, 许成才, 谷俊男, 任发政, 刘坤晓   

  1. 中国农业大学食品科学与营养工程学院 教育部-北京市功能乳品重点实验室; 中国农业大学食品科学与营养工程学院; 教育部-北京市功能乳品重点实验室; 北方交通大学附属中学;
  • 出版日期:2008-05-15 发布日期:2011-08-26

Effects of Different Factors on Chelating Activity of Casein Enzymatic Hydrolysate with Ferrous Salt

MAO  Xue-Ying, LI  Ming, LI  Min, XU  Shi-Ping, XU  Cheng-Cai, GU  Jun-Nan, REN  Fa-Zheng, LIU  Kun-Xiao   

  1. 1.College of Food Science and Nutritional Engineering,China Agricultural University,Key Laboratory of Functional Dairy,Ministry of Education and Beijing City;2.Affiliated Middle School of Northern Jiaotong University
  • Online:2008-05-15 Published:2011-08-26

摘要: 本研究以干酪素酪蛋白为原料,对影响酪蛋白酶解产物螯合Fe2+反应的因素进行了初步研究,为获得高效生物补铁剂以预防缺铁性贫血提供理论依据。研究表明,FeSO4与酪蛋白酶解产物的螯合能力显著高于(NH4)2Fe(SO4)2或FeCl2与酪蛋白酶解产物的螯合能力(p<0.05);不同酶解时间获得的酪蛋白酶解产物与FeSO4的螯合能力不同,7h时获得的酶解产物螯合FeSO4的能力最强;当酪蛋白酶解产物与FeSO4共同作用10min、硫酸亚铁与酪蛋白酶解产物的质量比为1:2、反应体系pH为6.0,反应温度为40℃时螯合效果较好。

关键词: 酪蛋白, 酶解产物, 亚铁, 螯合

Abstract: This study explored the effects of the kinds of ferrous salts,hydrolysis time,co-incubation time,mass ratio,reaction pH and temperature on the chelating activity of casein hydrolysate with ferrous salt,in order to prepare some basic data for preparation of a product rich in bio-iron.Peptides produced by enzyme hydrolysis of casein reacted with Fe2+.Results showed that the chelating activity of FeSO4 with casein hydrolysate was significantly higher than that of(NH4)2Fe(SO4)2 and FeCl2(p <0.05).The chelating activity of casein hydrolysate obtained at different hydrolysis time differed,and when hydrolysis time 7 h was the best.When the co-incubation time of casein hydrolysate and FeSO4,mass ratio,reaction pH and temperature were 10 min,1:2,6.0 and 40 ℃ respectively,the chelating effect was optimum.

Key words: casein, enzymatic hydrolysate, ferrous, chelate