关键词: 柳蒿芽, 免疫调节, 碳粒廓清, 溶血素, 淋巴细胞增殖

Abstract: Objective:To analyze the Artemisia bud main active ingredient qualitatively,and study Artemisia buds on immune modulatory effects.Method:Use the herbal classic qualitative test method,the solution of different polarity after the extraction tube reaction,and determine what the active ingredients contain in the Artemisia buds largely.Use untreated mice of different doses of Artemisia bud extract after intervention by carbon clearance experiment,chicken erythrocyte immunization,concanavalin A-induced lymphocyte proliferation test,evaluation Artemisia bud regulate immune function.Results:Artemisia buds main chemical ingredients are rough alkaloids,organic acids,proteins,polysaccharides,flavonoids and other substances.Normal control group,Artemisia bud extract high dose group of mice can increase carbon clearance index(p<0.01) and phagocytic index p<0.01),suggesting Artemisia bud extract may enhance normal mouse macrophage phagocytosis.It demonstrates that Artemisia buds can enhance the mouse nonspecific immune function.The hemolysin level and the lymphocyte proliferation capacity of the intervention of middle-dose and high-dose mice is significantly higher than the control group(p<0.01).It suggested that extract of Artemisia buds can be dissolved by increasing serum hemoglobin levels and enhance lymphocyte proliferative ability to enhance the special mice heterosexual immune function.Conclusion:Artemisia bud extract can increase the non-specific immune response and specific immune response of mice,so it can enhance immune system function.

Key words: Artemisia integrifolia L., immunity modulation, carbon clearance, hemolysin, lymphocyte proliferation