食品科学 ›› 2008, Vol. 29 ›› Issue (6): 142-145.

• 工艺技术 • 上一篇    下一篇

红茶菌主要功能菌株发酵糖茶水条件的研究

 杨莉, 籍保平, 周峰, 杨志伟, 李涛   

  1. 中国农业大学食品科学与营养工程学院; 中国农业大学食品科学与营养工程学院
  • 出版日期:2008-06-15 发布日期:2011-08-26

Study on Fermentation Conditions of Kombucha Key Functional Bacteria

 YANG  Li, JI  Bao-Ping, ZHOU  Feng, YANG  Zhi-Wei, LI  Tao   

  1. College of Food Science and Nutritional Engineering, China Agricultural University
  • Online:2008-06-15 Published:2011-08-26

摘要: 葡糖酸醋酸杆菌(Gluconacetobacter sp.A4)作为红茶菌中的主要功能菌株具有很强的产D-葡萄糖二酸1,4内酯(DSL)能力。本实验探讨了温度、pH、叶酸、木糖醇和发酵时间对A4生长及代谢产DSL的影响,得出最佳培养工艺:培养液组成为红茶0.5%(W/V)、葡萄糖5%(W/V)和木糖醇3%(W/V),接种量10%(V/V);在发酵过程中维持pH为4.0、发酵温度30℃,静止培养8d。发酵结束后,葡萄糖利用率达82%,DSL产量为4.69mg/ml。

关键词: 红茶菌, 葡糖酸醋酸杆菌, D-葡萄糖二酸1, 4内酯, 气相色谱检测法

Abstract: Gluconacetobacter sp. A4 (G. sp. A4), which has strong ability to produce D-saccharic acid 1, 4 lactone (DSL), is the key functional bacteria isolated from the kombucha preserved. This studay investigated the effect of temperature, pH, folic acid,xylitol and fermentation time on the growth of A4 and DSL production. The results showed that the optimum conditions are as follows: black tea 0.5% (W/V), glucose 5% (W/V), and xylitol 1.5% (W/V); the inoculation quantity 10% (V/V); pH maintaining at 4.0 and fermenting at 30 ℃ for 8 d. At the end of fermentation, the rate of glucose consumption is 82%, and DSL concentration reaches 4.69 mg/ml.

Key words: kombucha, Gluconacetobacter sp. A4, D-saccharic acid 1,4 lactone, gas chromatography