食品科学 ›› 2005, Vol. 26 ›› Issue (2): 105-108.

• 工艺技术 • 上一篇    下一篇

米根霉发酵甘薯淀粉制备L-乳酸研究

 潘丽军, 李兴江, 潘利华, 郑志, 余赟   

  1. 合肥工业大学生物与食品工程学院教育部农产品生物化工重点实验室
  • 出版日期:2005-02-15 发布日期:2011-09-19

Production of L-Lactic Acid from Sweet Potato Starch Fermented with Rhizopus oryzae

 PAN  Li-Jun, LI  Xing-Jiang, PAN  Li-Hua, ZHENG  Zhi, YU  Bin   

  1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230069, China; Key Laboratory of Bio-process, (Hefei University of Technology) Ministry of Education
  • Online:2005-02-15 Published:2011-09-19

摘要: 以米根霉AS3.819的诱变株为菌种,甘薯淀粉为碳源,在摇瓶发酵工艺的基础上,利用10L发酵罐对该菌株产L-乳酸进行研究,探索出10L罐最适发酵条件为:装液量40%,搅拌转速400~650r/min,通气流量0.5~0.6L/(L·min),溶氧≥88%,温度32℃,pH5.0~5.5,消泡剂添加量0.1%左右。罐发酵结果得到L-乳酸累积浓度为99.54g/L,对原淀粉的转化率达77%,最高生物量7.2g/L,发酵周期70h。

关键词: 米根霉, L-乳酸, 发酵, 甘薯淀粉, 溶氧

Abstract: A 10L jar fermentation technology for production of L-lactic acid from sweet potato starch incubated with a mutant of Rhizopus oryzae AS 3.819 was developed. The optimal culture conditions were as follows: 10L jar contained 4L liquid culture medium with rotational speed from 400 to 650r/min, aeration rate from 0.5 to 0.6L/(L·min), dissolved oxygen (DO) above 88%, fermentation temperature 32℃, pH from 5.0 to 5.5 and the dose of defoamer 0.1%. Results indicated that the average yield of L-lactic acid, the conversion rate of crude starch to L-lactic acid and top biomass fermentation time were 99.54g/L, 77%, 7.2g/L and 70h, respectively.

Key words: Rhizopus oryzae, L-lactic acid, sweet potato starch, DO