食品科学 ›› 2004, Vol. 25 ›› Issue (6): 160-162.

• 分析检测 • 上一篇    下一篇

发酵液中香兰素测定方法的研究

 黄艳凤, 周庆礼, 王昌禄   

  1. 天津科技大学食品与生物工程学院
  • 出版日期:2004-06-15 发布日期:2011-10-24

A Study for Determining Vanillin in Fermentation Broth

 HUANG  Yan-Feng, ZHOU  Qing-Li, WANG  Chang-Lu   

  1. College of Food Science and Biological Engineering,Tianjin Technology University
  • Online:2004-06-15 Published:2011-10-24

摘要: 对发酵液中香兰素的定性(薄层层析法)及定量方法(硫代巴比妥酸光度法和高效液相色谱法)进行了初步研究,结果表明,薄层层析法是一种很好的定性、半定量方法;硫代巴比妥酸光度法和高效液相色谱法具有良好的相关性和较高的准确度和精密度,说明除了高效液相色谱法外,硫代巴比妥酸光度法是一种既简单又准确的定量方法。

关键词: 香兰素, 薄层层析法, 硫代巴比妥酸光度法, 高效液相色谱法

Abstract: A pilot study for determination of vanillin in fermentation broth with the TLC method and TBA spectrophotometeryand HPLC method has been carried out. It was found that TLC method could be used to quantitative analysis. The experimentalso showed that TBA spectrophotometery, HPLC method have a good relationship, and could be reproducible. Besides theHPLC method, the TBA spectrophotometery was simple and practical for determining vanillin in the fermentation broth.

Key words: vanillin, TLC, TBA spectrophotometery, HPLC