食品科学 ›› 2003, Vol. 24 ›› Issue (12): 52-54.

• 基础研究 • 上一篇    下一篇

嗜酸乳杆菌产生β-半乳糖苷酶活力的测定及数学模型的建立

 赵瑞香, 孙俊良, 于涛   

  1. 河南职业技术师范学院食品学院
  • 出版日期:2003-12-15 发布日期:2011-12-13

Activity Assay on β-galactosidase Produced by L.acidophilus In-1 and L.acidophilus Lakcid

 ZHAO  Rui-Xiang, SUN  Jun-Liang, YU  Tao   

  1. College of Food, Henan Vocational Normal University
  • Online:2003-12-15 Published:2011-12-13

摘要: 以MRS为培养基,对L.acidophilus Ind-1和L.acidophilus Lakcid 两菌株产生β-半乳糖苷酶的活力进行了研究。结果表明,采用1%SDS:氯仿=1:10细胞破壁β-半乳糖苷酶的提取率较高,37℃ 36h培养,L.acidophilusInd-1和L.acidophilus Lakcid 可以产生 2.229μ moleONP/L.min 和 1.808μmoleONP/L.min的β-半乳糖苷酶,且该酶在MRS培养基中积累的数学模型为 Logistic和Mistcherlich方程。

关键词: 嗜酸乳杆菌, &beta, -半乳糖苷酶, 数学模型

Abstract: The activity of β-galactosidase produced by both L.acidophilus Ind-1 and L.acidophilus Lakcid was studied byusing MRS as culture medium. The results showed that the extraction rate of β-galactosidase was higher using 1%SDS:chloroform=1:10 to break the cell wall; that 2.229μ moleONP/L.min and 1.808μmoleONP/L.min β-galactosidase were formedby the two stains after 36h culture at 37℃;and that the math models of this enzyme accumulated in MRS were Logistic andMistcherlich.

Key words: Lactobacillus acidophilus, β-galactosidase, math model