食品科学 ›› 2008, Vol. 29 ›› Issue (11): 305-307.

• 工艺技术 • 上一篇    下一篇

龙牙百合多糖的纯化及其分子量的测定

 陈小蒙, 刘成梅, 刘伟   

  1. 南昌大学食品科学与技术国家重点实验室; 南昌大学中德食品工程中心;
  • 出版日期:2008-11-15 发布日期:2011-12-08

Purification and Molecular Weight Determination of Longya lilium Polysaccharide

 CHEN  Xiao-Meng, LIU  Cheng-Mei, LIU  Wei   

  1. 1.State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;2.Sino-German Food Engineering Center,Nanchang university,Nanchang 330047,China
  • Online:2008-11-15 Published:2011-12-08

摘要: 本研究用DEAE-sepharose Fast Flow柱从百合水提物中分离纯化得到两种多糖LLP1、LLP2,经高效凝胶渗透色谱法(HPGPC)鉴定LLP1为单一组分、LLP2为糖蛋白,将这两种多糖进行气相色谱分析。结果如下:LLP1由甘露糖、葡萄糖和阿拉伯糖组成,分子量为11756D,LLP2由半乳糖、鼠李糖和阿拉伯糖组成,分子量1038773D。

关键词: 龙牙百合多糖, 纯化, 分子量, HPGPC

Abstract: Two polysaccharides(LLP1 and LLP2) were isolated from Longya lilium water extract with DEAE-Sepharose Fast Flow column.They are identified as pure polysaccharide and glycoprotein by HPGPC,respectively.The monosaccharide composi-tions of LLP1 consist of Man,Glu and Ara,and its molecular weight is 11756 D.The monosaccharide compositions of LLP2 are Gal,Rha and Ara and its molecular weight is 1038773 D.

Key words: Longya lilium polysaccharide(LLP), purification, molecular weight, HPGPC