食品科学 ›› 2006, Vol. 27 ›› Issue (12): 50-53.

• 基础研究 • 上一篇    下一篇

粗壮脉纹孢菌所产纤维素酶的性质研究

余玮,邓泽元,范亚苇,余永红,吴丹   

  1. 南昌大学食品科学与工程系 食品科学教育部重点实验室;
  • 出版日期:2006-12-15 发布日期:2011-11-23

Study on the Properties of Cellulase in Neurospora crassa

YU Wei,DENG Ze-yuan,FAN Ya-wei,YU Yong-hong,WU Dan   

  1. Key Laboratory of Food Science, Ministry of Education, Department of Food Science and Engineering, Nanchang University, Nanchang 330047, China
  • Online:2006-12-15 Published:2011-11-23

摘要: 本文对粗壮脉纹孢菌(Neurosporacrassa)所产纤维素酶的一般酶学性质进行研究,得出的结论如下:该酶的最适催化反应pH为4.8~5.3,最适催化反应温度为45~50℃,在50℃及pH为4.8时该酶稳定。CMC、C1和β-葡萄糖苷酶活力变化范围有一定差别。锰离子对该酶活力有一定的增强作用,而铜离子和铝离子对其活力有明显的抑制作用。在产酶培养基中加入洗衣粉可以促进产纤维素酶活力,而洗洁精则会抑制产酶活力。

关键词: 粗壮脉纹孢菌(Neurosporacrassa), 纤维素酶, 酶性质

Abstract: The general properties of cellulase in Neurospora crassa was studied in this paper. The results were as follows: the optimal conditions of enzymatic hydrolysis were at pH4.8~5.3, 45~50℃. Cellulase was stable at pH4.8 and 50℃, and the activity of CMCase, Clase and β-glucosidase were different from one another. The cellulase was activated by Mn2+, but inhibited by Cu2+ and Al3+. Adding washing powder into producting enzyme substrate could improve the activity of cellulase, but detergent liquid could inhibit the activity.

Key words: Neurospora crassa, cellulase, properties of enzyme