磁性固定化纤维素酶的制备及其磁响应性

食品科学 ›› 2006, Vol. 27 ›› Issue (12): 335-339.

磁性固定化纤维素酶的制备及其磁响应性

寇灵梅，李冰，郭祀远，李琳，黄彩坚

华南理工大学轻工与食品学院轻化工研究所；

出版日期:2006-12-15 发布日期:2011-11-23

Preparation of Magnetic Immobilized Cellulase and Its Characteristics under External Magnetic Field

KOU Ling-mei,LI Bing,GUO Si-yuan,LI Lin,HUANG Cai-jian

College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, China

Online:2006-12-15 Published:2011-11-23

摘要/Abstract

摘要： 本研究以磁性壳聚糖微球为载体,采用吸附法制备磁性固定化纤维素酶。以酶活力为考察指标,研究了不同工艺条件对制备固定化酶的影响,及其在外加磁场下的磁响应性的变化。实验结果表明,制备磁性固定化纤维素酶最适条件为:吸附时间10h,pH5.0,离子浓度0.05mol/L,酶用量为150mg。在外加磁场作用下,比较磁性固定化纤维素酶与游离纤维素酶酶活力的变化发现:当磁场强度<500mT与曝磁时间<60min时,游离纤维素酶的酶活随着磁场强度与曝磁时间的增加而增加,但超出此范围,酶活就明显下降;而当磁场强度<300mT与曝磁时间<60min时,磁性固定化纤维素酶酶活与游离纤维素酶的变化趋势相似。

关键词: 磁性固定化纤维素酶, 酶活力, 外加磁场, 磁响应性, 磁场强度, 曝磁时间

Abstract: Magnetic immobilized enzymes are widely employed in magnetically stabilized fluidized bed reactor. The magnetic immoblized cellulases were prepared by immobilized cellulases on magnetic chitosan microspheres using the physical adsorp-tion method, and their magnetic responsiveness was investigated. The results demonstrated the optimum conditions of prepar-ing immobilized cellulase were: pH5.0, buffer solution concentration of 0.05mol/L, the amount of cellulase used of 150mg, immobilization time of 10h. And the activities of the magnetic immobilized cellulase and free cellulase exposed to the external magnetic field were measured. It was indicated that the activities of free cellulase increased with the increasing of magnetic field intensity when the intensity was less than 500mT and the exposure time was less than 60min, however, out of this range, a sharp decrease on the activities was detected. And the change of the activities of magnetic immobilized cellulases showed the same trend as that of free cellulases.

Key words: magnetic immobilized cellulase, enzyme activity, external magnetic field, magnetic responsiveness, magnetic field intensity, exposure time

寇灵梅，李冰，郭祀远，李琳，黄彩坚. 磁性固定化纤维素酶的制备及其磁响应性[J]. 食品科学, 2006, 27(12): 335-339.

KOU Ling-mei,LI Bing,GUO Si-yuan,LI Lin,HUANG Cai-jian. Preparation of Magnetic Immobilized Cellulase and Its Characteristics under External Magnetic Field[J]. FOOD SCIENCE, 2006, 27(12): 335-339.

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