赤芍总苷的分离提取和纯化研究

食品科学 ›› 2006, Vol. 27 ›› Issue (12): 354-360.

赤芍总苷的分离提取和纯化研究

莫晓燕，朴田，赵文娟

西安交通大学生命科学与技术学院；

出版日期:2006-12-15 发布日期:2011-11-23

Study on Extraction and Purification of Total Paeony Glycoside

MO Xiao-yan,PIAO Tian,ZHAO Wen-juan

School of Life Science and Technology, Xia’n Jiaotong University, Xia’n 710049, China

Online:2006-12-15 Published:2011-11-23

摘要/Abstract

摘要： 采用天然产物分离提取纯化技术对赤芍总苷的提取和纯化方法进行了研究,通过单因子-正交设计法,得到的提取工艺条件分别为:乙醇浓度70%,提取时间30min,料液比1:10,加热回流提取2次,赤芍总苷得率为4.68%;乙醇浓度70%,提取时间30min,料液比1:14,常温下超声提取2次,赤芍总苷得率为4.47%。此外,采用五种大孔吸附树脂富集纯化提取液中的赤芍总苷,实验结果表明,非极性树脂D101和ATD-21是性能良好的赤芍总苷吸附剂。ATD-21型树脂的最佳吸附及解吸条件为:上样浓度2.1～4.2mg/ml,吸附流速1.0～3.0ml/min,最大上样量52.5mg/g树脂,最佳洗脱剂为30%的乙醇60ml。用此条件纯化赤芍总苷提取液的收率为82.9%,纯度为74.1%。

关键词: 赤芍总苷, 提取, 纯化, 大孔吸附树脂

Abstract: With the single-factor experiment and the orthogonal design, various extracting conditions for total paeony glycoside (TPG) were observed. The results showed that the optimum extracting conditions of ethanol under reflux was 70% ethanol with 10 times of solvent to extract twice, once for 30min, and the extraction rate of TPG was 4.68%; the best technique of supersonic wave extraction was 70% ethanol with 14 times of solvent to extract twice, once for 30min, the extraction rate of TPG was 4.47%. Also, the purification of TPG by macro adsorption resin was investigated. The results showed that nonpolar resin ATD-21 and D101 were good sorbents of TPG. The optimum conditions of ATD-21 included that the concentration of sample was 2. 1～4.2mg/ml with flow rate of 1.0～3.0ml/min, the quantity of sample was 52.5mg/g resin and 30% ethanol 60ml was used as eluant. With the optimum technological process, the collection ratio of TPG was 82.9% and the purity reached 74.1%.

Key words: total paeony glycoside (TPG), extraction, purification, macro adsorption resin

莫晓燕，朴田，赵文娟. 赤芍总苷的分离提取和纯化研究[J]. 食品科学, 2006, 27(12): 354-360.

MO Xiao-yan,PIAO Tian,ZHAO Wen-juan. Study on Extraction and Purification of Total Paeony Glycoside[J]. FOOD SCIENCE, 2006, 27(12): 354-360.

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