食品科学 ›› 2006, Vol. 27 ›› Issue (12): 428-432.

• 工艺技术 • 上一篇    下一篇

产乳糖酶酵母Kluyveromyces lactis培养产酶发酵条件的研究

张明丽,刘文群,熊华,陈望华,肖芳,简霜泉   

  1. 南昌大学食品科学教育部重点实验室;
  • 出版日期:2006-12-15 发布日期:2011-11-23

Fermentation Conditions Study of Lactase-Producing Yeast Kluyveromyces lactis

ZHANG Ming-li,LIU Wen-qun,XIONG Hua,CHEN Wang-hua,XIAO Fang,JIAN Shuang-quan   

  1. Key Laboratory of Food Science, Ministry of Education, Nanchang University, Nanchang 330047, China
  • Online:2006-12-15 Published:2011-11-23

摘要: 通过正交试验和单因素试验确定了本实验室乳酸克鲁维酵母菌株产乳糖酶的发酵培养基组分(W/V,%)为乳糖8、葡萄糖0.5、酵母膏0.7、尿素0.15、KH2PO41.5、MgSO40.1、MnCl20.01,并考察了其发酵工艺条件,优化得到的最高产酶量平均达到1.930ONPGU/ml。

关键词: 乳糖酶, 乳酸克鲁维酵母, 发酵条件

Abstract: The fermentation culture medium of lactase-producing yeast Kluyveromyces lactis was optimized by orthogonal design and single-factor experiments. The optimal culture medium for fermentation of lactase-producing yeast Kluyveromyces lactis was shown as follows (%, W/V): lactose 8, glucose 0.5, yeast extract 0.7, urea 0.15, KH2PO4 1.5, MgSO4 0.1, MnCl2 0.01. The conditions of fermentation were also investigated in the trail and the average productivity of lactase was 1.930 ONPG unitsp erm illiliter.

Key words: lactase, Kluyveromyces lactis, fermentation conditions