食品科学 ›› 2006, Vol. 27 ›› Issue (12): 432-436.

• 工艺技术 • 上一篇    下一篇

油菜花多糖的提取及纯化研究

孙汉巨,潘丽军,靳锁   

  1. 合肥工业大学生物与食品工程学院;
  • 出版日期:2006-12-15 发布日期:2011-11-23

Study on Isolation and Purification of Polysaccharides from Flower of Rape

SUN Han-ju,PAN Li-jun,JIN Suo   

  1. College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China
  • Online:2006-12-15 Published:2011-11-23

摘要: 对油菜花多糖的提取工艺、分离纯化和分子量测定作了研究。通过单因素实验,考察了影响多糖得率的因素;通过正交试验进一步优化提取工艺条件,得出油菜花多糖最佳提取条件是液料比为40,提取温度为100℃,提取时间为1.5h,提取次数为2次。通过凝胶柱层析法对粗多糖进行进一步纯化和分子量的测定,结果表明:油菜花多糖主要含有两种组分,其分子量分别为63.51kD和49.58kD。

关键词: 油菜花, 多糖, 提取, 纯化

Abstract: The extraction and purification of the rape polysaccharides were studied .The factors that affected the extracting rates were reviewed by experiments of mono-factors. Then, adopting the orthogonal test, the optimum extraction conditions were obtained. The ratio of liquid to raw material, extraction time, extraction temperature and extraction times were 40, 2h, 100℃ and 2, respectively. The size-exclusion column chromatography was used to purify crude polysaccharides and determine their molecular weights .The results showed the rape polysaccharides were composed of two pure polysaccharides, which molecular weights were 63.51kD and 49.58kD, respectively.

Key words:  , flower of rape; polysaccharide; extraction; purification;