食品科学 ›› 2006, Vol. 27 ›› Issue (12): 518-520.

• 工艺技术 • 上一篇    下一篇

生物酶法提取葡萄籽油的工艺研究

刘颖,周利娟,王欣   

  1. 哈尔滨商业大学食品工程学院;
  • 出版日期:2006-12-15 发布日期:2011-11-23

Study on Technology of the Enzymatic Extraction Oil for Grape Seed Oil

 LIU  Ying, ZHOU  Li-Juan, WANG  Xin   

  1. College of Food Engineering, Harbin University of Commerce, Harbin 150076, China
  • Online:2006-12-15 Published:2011-11-23

摘要: 生物酶法提取植物油脂是一种新型的油脂加工方法,它既可提高油脂的提取率,又可获得品质较优的植物油脂。以葡萄籽为原料分别加入纤维素酶和中性蛋白酶进行酶解,通过试验分别得出两种酶提取葡萄籽油的最佳工艺参数:纤维素酶提取葡萄籽油的最佳工艺参数为:酶解温度为45℃、酶解时间为1.5h、酶解pH值为4.5、酶用量为3000U/g葡萄籽,油脂提取率为84.1%;中性蛋白酶提取葡萄籽油的最佳工艺参数为:酶解温度为55℃、酶解时间为2.0h、酶用量为2000U/g葡萄籽,油脂提取率为80.3%。对两种酶解提油工艺进行比较可知:中性蛋白酶提油工艺所需成本较低,提取率较高,适合于葡萄籽油的提取。

关键词: 葡萄籽油, 纤维素酶, 中性蛋白酶, 有机溶剂萃取

Abstract: Enzyme extraction of plant oil was a new oil processing method. In the case of oil extraction process, two advantages were offered by this treatment: higher yields of oil and higher quality of the oil. In this thesis the raw material was grape seed and then the grape seed was hydrolyzed by cellulase and neutral protease respectively. From the experiment we got the optimum conditions of extraction oil by cellulase and the optimum technology parameters were: temperature: 45℃, time: 1.5h, pH: 4.5, concentration: 3000U per gram grape seed, the extraction rate was 84.1% ; The optimum conditions of extraction oil by neutral protease and the optimum technology parameters were: temperature: 55℃, time: 2.0h, concentration: 2000U per gram grape seed, the extraction rate was 80.3%. Compared with the cellulase hydrolysis, the neutral protease hydrolysis was suitably used to extract oil from grape seed because its cost was lower than cellulase hydrolysis.

Key words: grape seed oil, cellulase, protease, solvent extraction