食品科学 ›› 2006, Vol. 27 ›› Issue (12): 634-636.

• 分析检测 • 上一篇    下一篇

高效液相色谱法测定柑橘汁中类胡萝卜素

胡建中,王可兴,潘思轶   

  1. 华中农业大学食品科学技术学院;
  • 出版日期:2006-12-15 发布日期:2011-11-23

Separation and Determination of Carotenoids in Orange Juice by HPLC

 HU  Jian-Zhong, WANG  Ke-Xing, PAN  Si-Yi   

  1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
  • Online:2006-12-15 Published:2011-11-23

摘要: 用正己烷、丙酮、乙醇混合溶剂提取宽皮柑橘大叶尾张中类胡萝卜素,10%KOH的甲醇溶液皂化12h,比较了hypersilODS2和YMCC30两种不同的色谱柱的分离效果。结果表明C30色谱柱分离效果优于hypersilODS2色谱柱。通过类胡萝卜素典型的特征吸收峰,结合在线的PAD检测器,初步判断在C30色谱柱分离的类胡萝卜素有18种。

关键词: 柑橘, 类胡萝卜素, 色素, HPLC

Abstract: High-performance liquid chromatography with photodiode array detection was applied for separation and characterization of carotenoids from Owari. The carotenoid pigments were extracted using hexane/acetone/ethanol and saponi-fied using 10% methanolic potassium hydroxide. Two different columns were compared to determine carotenoids, more than 18 carotenoid pigments were separated with YMC C30 column.

Key words: citrus, carotenoid, pigment, HPLC