蛋氨酸螯合铁对大鼠肝脏物质代谢相关基因表达的影响

食品科学 ›› 2006, Vol. 27 ›› Issue (12): 735-739.

蛋氨酸螯合铁对大鼠肝脏物质代谢相关基因表达的影响

王立宽，乐国伟，顾博，李亮，施用晖

江南大学食品科学与安全教育部重点实验室；江南大学食品学院食品营养与安全研究所；

出版日期:2006-12-15 发布日期:2011-11-23

Effect of Methionine Chelate Iron on the Liver Gene Expression and Metabolism of Rat

WANG Li-kuan1,2,LE Guo-wei1,2,GU Bo2,LI Liang2,SHI Yong-hui

1.Key Laboratory of Food Science and Security, Ministry of Education, Southern Yangtze University, Wuxi 214036, China;2.Institute of Food Nutrition and Saftey, School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China

Online:2006-12-15 Published:2011-11-23

摘要/Abstract

摘要： 研究缺铁日粮中补充蛋氨酸螯合铁与硫酸亚铁对大鼠铁利用及肝基因表达的影响。20只SD断奶大鼠,建立缺铁大鼠模型,然后均分成两组,分别饲喂加入等量铁(35mg/kg)的蛋氨酸螯合铁和硫酸亚铁日粮,补铁后4、7d后测定体重、血红蛋白,组织器官铁含量及血清总铁结合力;并提取大鼠肝组织总RNA,用Afymetrix基因芯片进行基因表达分析,研究硫酸亚铁与蛋氨酸螯合铁对缺铁大鼠物质代谢基因表达的差异。结果表明,蛋氨酸螯合铁组大鼠增重明显高于硫酸亚铁组,血红蛋白、总铁结合力含量有升高趋势。与硫酸亚铁组比较,蛋氨酸螯合铁组肝组织珠蛋白与转铁蛋白合成相关基因,以及胆固醇合成,脂肪代谢、ATP合成相关基因明显上调。补充蛋氨酸螯合铁动物铁代谢和物质代谢存在显著差异。

关键词: 大鼠, 蛋氨酸螯合铁, 基因表达, 代谢

Abstract: Using Affymetrix genechip to analyse the differential gene expression in rats which fed with ferrous methionine chelate and ferrous sulfate respectivly. To found a model of anaemic rat which dietary were iron deprivation for 15 days, then 20 SD rats were divided randomly into two groups. The rats were fed with same iron level (Fe 35mg/kg) of ferrous methionine chelate and ferrous sulfate respectively. After 4th and 7th day of iron supplementation, rats gain, the contents of hemoglobin (Hb) and serum total iron-binding capacity of rats were measured. Gene expression of liver were detected by highdensity oligonuleotide array and compared ferrous methionine chelate group with ferrous sulfate group at the 7th day. The body gains, contents of hemoglobin (Hb) and serum total iron-binding capacity of rats fed with ferrous methionine chelate were higher than ferrous sulfate group. Ferrous methionine chelate induced upregulated expressed genes involved globin (Hbg1), ferric transfer protein (FTL1), en-zymes of cholesterol synthesize, fatty acid synthesize and ATP synthesase in liver. These findings may thus explain different functions between ferrous methionine chelate and ferrous sulfate.

Key words: rat, ferrous methionine chelate, differential genes expression, microarray

王立宽，乐国伟，顾博，李亮，施用晖. 蛋氨酸螯合铁对大鼠肝脏物质代谢相关基因表达的影响[J]. 食品科学, 2006, 27(12): 735-739.

WANG Li-kuan1,2,LE Guo-wei1,2,GU Bo2,LI Liang2,SHI Yong-hui. Effect of Methionine Chelate Iron on the Liver Gene Expression and Metabolism of Rat[J]. FOOD SCIENCE, 2006, 27(12): 735-739.

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