软枣猕猴桃多糖体外抗氧化活性

食品科学 ›› 2012, Vol. 33 ›› Issue (23): 79-82.

软枣猕猴桃多糖体外抗氧化活性

刘长江，潘松，梁爽

沈阳农业大学食品学院

收稿日期:2011-10-07 修回日期:2012-11-09 出版日期:2012-12-15 发布日期:2012-12-12
通讯作者: 刘长江 E-mail:liucj597@sohu.com
基金资助:
农业部公益性行业（农业科研专项）东北野生猕猴桃资源保护、开发与利用

in vitro Antioxidant Activity of Polysaccharides from Actinidia arguta Sieb. et Zucc. Fruits

Received:2011-10-07 Revised:2012-11-09 Online:2012-12-15 Published:2012-12-12

摘要/Abstract

摘要： 利用水提醇沉法提取软枣猕猴桃粗多糖，经脱蛋白、脱脂，软枣猕猴桃粗多糖的提取率为1.41%。利用DEAE-52纤维素离子交换层析对软枣猕猴桃多糖进行初步分离，得到4种软枣猕猴桃多糖组分。利用Sephadex G-200凝胶柱层析对组分Ⅱ和Ⅲ实现了完全分离。通过测定清除自由基能力，评价软枣猕猴桃多糖组分Ⅱ的抗氧化活性。结果表明：软枣猕猴桃多糖对DPPH自由基及烷基自由基(•R)有较强的清除能力，IC50值分别为0.497、0.547mg/mL。在受试范围内，随软枣猕猴桃多糖质量浓度的增加，清除能力增强，当软枣猕猴桃多糖质量浓度达到1mg/mL时，其对DPPH自由基及•R的清除率分别达到86.4%、87.1%，与VC的清除率相近。软枣猕猴桃多糖对羟自由基(•OH)有一定的清除能力，IC50值为0.668mg/mL。其清除能力随多糖质量浓度的增加而有所增加，但其对•OH的清除率较VC有一定的差距。软枣猕猴桃多糖对O2－·的清除率较低，清除能力较弱。由此可见，软枣猕猴桃多糖具有较强的抗氧化活性。

关键词: 软枣猕猴桃, 多糖, 抗氧化活性, 自由基, 清除率

Abstract: Crude polysaccharides from Actinidia arguta Sieb. et Zucc. fruits were extracted by hot water extraction and ethanol precipitation, deproteinized and defatted and the yield of crude polysaccharides was found to be 1.41%. The crude polysaccharide extract was separated by DEAE-cellulose 52 ion exchange chromatography to obtain four fractions (Ⅰ, Ⅱ, Ⅲ and Ⅳ). Complete separation of fractions Ⅱ and Ⅲ was achieved by Sephadex G-200 column chromatography. The antioxidant activity of fraction Ⅱ was investigated by measuring free radical scavenging activity. Polysaccharide fraction II was found to have strong radical scavenging activity against DPPH and alkyl radicals with an IC50 of 0.497 mg/mL and 0.547 mg/mL, respectively. Its radical scavenging activity increased with increasing concentration within the concentration range investigated and the scavenging rate at a concentration of 1 mg/mL was 86.4% and 87.1% for DPPH and alkyl radicals, respectively, which was comparable to that of vitamin C. Moreover, this fraction also had radical scavenging activity against hydroxyl radical with an IC50 of 0.668 mg/mL in a concentration-dependent manner but was inferior to vitamin C. However, its radical scavenging activity against superioxide anion radical was weak. Based on the above results, we conclude that polysaccharide fraction Ⅱ has potent antioxidant activity.

Key words: Actinidia arguta Sieb. et Zucc., polysaccharides, antioxidant activity, free radical, scavenging rate

中图分类号:

TS201.1

刘长江，潘?松，梁?爽. 软枣猕猴桃多糖体外抗氧化活性[J]. 食品科学, 2012, 33(23): 79-82.

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