盐生海芦笋来源真菌Penicillium stoloniferum发酵产物抗肿瘤活性成分研究

摘要/Abstract

摘要： 采用活性追踪的方法，对一株盐生海芦笋来源真菌Penicillium stoloniferum发酵产物中的抗肿瘤活性成分进行分离和鉴定。利用常压硅胶柱层析、Sephedex LH-20、半制备HPLC等分离方法从中分离得到8个单体化合物，通过理化性质分析及波谱学方法鉴定其化学结构分别为：麦角甾-7,9(11),22-三烯-3-醇、麦角固醇、菠菜甾醇、5α,8α-环二氧-(22E,24R)-23-甲基麦角甾-6,22-二烯-3β-醇、色氨酸-苯丙氨酸-环二肽、苯丙氨酸、邻苯二甲酸正丁酯、邻苯二甲酸异丁酯；以SRB法评价化合物的抗肿瘤活性，5α,8α-环二氧-(22E、24R)-23-甲基麦角甾-6,22-二烯-3β-醇、邻苯二甲酸正丁酯、邻苯二甲酸异丁酯对小鼠乳腺癌P388细胞具有强的细胞毒活性，IC50值分别为14、34、46μg/mL，其他化合物没有活性。

关键词: 盐生海芦笋真菌, 发酵产物, 结构鉴定, 抗肿瘤活性

Abstract: Eight anti-tumor monomer compounds were isolated from the fermented broth and mycelia of the fungus Penicillium stoloniferum derived from Salicornia herbacea by bioassay-guided isolation method using silica gel column chromatography, Sephedex LH-20 chromatography, and semi-preparative HPLC. They were identified by physiochemical analysis and spectroscopic techniques as ergosta-7,9(11),22-trien-3-ol, ergosterol, spinasterol, 5α,8α-epidioxy-(22E,24R)-23-methylergosta-6,22-dien-3β-ol, tryptophan phenylalanine cyclic dipeptide, phenylalanine, dibutyl phthalate and diisobutyl phthalate. Only 5α,8α-epidioxy-(22E,24R)-23-methylergosta-6,22-dien-3β-ol, dibutyl phthalate and diisobutyl phthalate had strong cytotoxicity with IC50 values of 14, 34 μg/mL and 46 μg/mL, respectively, as evaluated by SRB method.

Key words: Salicornia herbacea fungi, fermentation products, structure identification, anti-tumor activity

中图分类号:

TS201.2

沈可慧，孟晓露，刘天行，辛志宏*. 盐生海芦笋来源真菌Penicillium stoloniferum发酵产物抗肿瘤活性成分研究[J]. 食品科学, 2012, 33(23): 278-282.

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