关键词: 杏鲍菇, 菇头, 多糖, 抗氧化活性, 抗肿瘤活性, 结构

Abstract: Crude polysaccharide (CP) was obtained by hot water extraction from the stalk residue of Pleurotus eryngii. The molecular mass distribution of CP was assayed by the combined use of gel permeation chromatography (GPC), differential refraction detector (RID), and multi-angle light scattering (MALLS). The monosaccharide composition was assayed by gas chromatography (GC). The glycosyl linkages were analyzed by gas chromatography-mass spectrometry. The antioxidant activity was measured by the 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical cation assay. The antitumor activity was assayed by both in vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and in vivo methods. Results showed that glucose was the predominant monosaccharide (84.4%) of CP; the molecular mass of CP was widely distributed and mainly composed of 4 peaks (i.e. 3.816 × 107, 2.010 × 106, 4.572 × 105 and 1.849 × 105 Da). The glycosyl linkages mainly consisted of 1,3-linked (42.7%) and 1,6-linked (35.3%) glucose residues. At a concentration of 5 mg/mL, the crude polysaccharides exerted 70.9% scavenging rate against ABTS radical cation. In addition, the in vivo test showed that CP could significantly inhibit 39.84% of the growth of transplantable U14 cervical cancer in mice at a dose of 200 mg/(kg·d) after 12 days of intragastric administration.

Key words: Pleurotus eryngii, stalk residue, polysaccharide, antioxidant activity, antitumor activity, structure