荧光定量PCR在大肠杆菌O157:H7检测中的应用研究进展

doi:10.7506/spkx1002-6630-201311075

食品科学 ›› 2013, Vol. 34 ›› Issue (11): 358-362.doi: 10.7506/spkx1002-6630-201311075

荧光定量PCR在大肠杆菌O157:H7检测中的应用研究进展

王力均，许恒毅*，熊勇华，李萍

南昌大学食品科学与技术国家重点实验室，江西南昌 330047

收稿日期:2012-03-06 修回日期:2013-05-02 出版日期:2013-06-15 发布日期:2013-06-03
通讯作者: 许恒毅 E-mail:kidyxu@163.com
基金资助:
食品科学与技术国家重点实验室青年骨干研究基金项目

Application of Real-time Fluorescent Quantitative Polymerase Chain Reaction in Detection of Escherichia coli O157:H7

WANG Li-jun，XU Heng-yi*，XIONG Yong-hua，LI Ping

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China

Received:2012-03-06 Revised:2013-05-02 Online:2013-06-15 Published:2013-06-03
Contact: XU Heng-Yi E-mail:kidyxu@163.com
Supported by:
Research Foundation for Young Scientists of State Key Laboratory of Food Science and Technology

摘要/Abstract

摘要：

荧光定量PCR技术因具有较高的灵敏度、较好的重复性以及较强的特异性而在大肠杆菌O157:H7检测中广泛应用。本文对荧光定量PCR技术进行简介，并综述近年来其在大肠杆菌O157:H7检测中的应用概况，为基于荧光定量PCR方法检测大肠杆菌O157:H7提供一定的参考。

关键词: 荧光定量PCR, 大肠杆菌O157:H7, 检测, 研究进展

Abstract:

Fluorescent quantitative polymerase chain reaction (qPCR) is widely used for the detection of Escherichia coli
O157:H7 with high sensitivity, repeatability and specificity. In this article, qPCR is briefly introduced, and the applications
of qPCR in detection of E. coli O157:H7 during the past 10 years are reviewed.

Key words: fluorescent quantitative polymerase chain reaction, Escherichia coli O157:H7, detection, research progress

中图分类号:

王力均，许恒毅*，熊勇华，李萍. 荧光定量PCR在大肠杆菌O157:H7检测中的应用研究进展[J]. 食品科学, 2013, 34(11): 358-362.

WANG Li-jun，XU Heng-yi*，XIONG Yong-hua，LI Ping. Application of Real-time Fluorescent Quantitative Polymerase Chain Reaction in Detection of Escherichia coli O157:H7[J]. FOOD SCIENCE, 2013, 34(11): 358-362.

参考文献

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荧光定量PCR在大肠杆菌O157:H7检测中的应用研究进展

Application of Real-time Fluorescent Quantitative Polymerase Chain Reaction in Detection of Escherichia coli O157:H7

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