响应面法优化超声波辅助提取仿栗籽油工艺及其脂肪酸组成分析

食品科学 ›› 2012, Vol. 33 ›› Issue (16): 24-29.

响应面法优化超声波辅助提取仿栗籽油工艺及其脂肪酸组成分析

麻成金1,2，黄伟2，黄群2，吴竹青2，吴丽雅2

1.吉首大学林产化工工程湖南省重点实验室 2.吉首大学食品科学研究所

收稿日期:2012-04-10 修回日期:2012-07-11 出版日期:2012-08-25 发布日期:2012-09-07
通讯作者: 麻成金 E-mail:Machengjin368@126.com

Response Surface Optimization of Ultrasonic-Assisted Extraction and Fatty Acid Composition Analysis of

Received:2012-04-10 Revised:2012-07-11 Online:2012-08-25 Published:2012-09-07

摘要/Abstract

摘要： 采用超声波辅助提取仿栗籽油，通过单因素试验和响应面法(RSM)对提取工艺进行优化，并利用气相色谱-质谱联用法测定仿栗籽油的脂肪酸组成。结果表明，超声波辅助提取仿栗籽油的优化工艺条件为以环己烷为提取溶剂、超声工作/间歇时间为3s/1s、超声功率540W、超声时间18min、提取温度60℃、液料比8.6:1(g/mL)，在此工艺条件下，仿栗籽油提取率可达94.53%。气相色谱-质谱联用测定结果表明仿栗籽油中富含不饱和脂肪酸，总含量达到70.13%，其中油酸和亚油酸的含量分别为53.95%、16.18%。

关键词: 仿栗籽油, 超声波辅助提取, 响应面分析, 气相色谱-质谱联用分析, 脂肪酸组成

Abstract: Response surface methodology (RSM) was employed to optimize the ultrasound-assisted extraction of essential oil from Sloanea hemsleyana seeds. Meanwhile, the chemical composition of the extracted essential oil was analyzed by GC-MS. The results showed that the optimal extraction conditions were cyclohexane as the best extraction solvent, single ultrasonic treatment for 3 s at a power level of 540 W followed by a 1-s interval, 18 min of total extraction time, and 1:8.6 of solid-to-liquid ratio, resulting in an oil yield of 94.53%. The essential oil obtained under these conditions was abundant in unsaturated fatty acids with a total content of 70.13%. The contents of oleic acid and linoleic acid were 53.95% and 16.18%, respectively.

Key words: Sloanea hemsleyana seed oil, ultrasonic-assisted extraction, response surface analysis, GC-MS；fatty acid composition

麻成金1,2，黄伟2，黄群2，吴竹青2，吴丽雅2. 响应面法优化超声波辅助提取仿栗籽油工艺及其脂肪酸组成分析[J]. 食品科学, 2012, 33(16): 24-29.

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