刺梨三萜对人肝癌SMMC-7721细胞增殖的影响

doi:10.7506/spkx1002-6630-201313058

食品科学

刺梨三萜对人肝癌SMMC-7721细胞增殖的影响

黄姣娥1，江晋渝2，罗勇2，戴支凯1,*

1.桂林医学院药学院药理学教研室，广西桂林 541004；2.贵州航天医院神经内科，贵州遵义 563000

出版日期:2013-07-15 发布日期:2013-06-28
通讯作者: 戴支凯

Effect of Rosa roxburghii Tratt Triterpene on Proliferation of Human Hepatoma SMMC-7721 Cells

HUANG Jiao-e1，JIANG Jin-yu2，LUO Yong2，DAI Zhi-kai1,*

1. Department of Pharmacology, Pharmaceutical Institute of Guilin Medical University, Guilin 541004, China；
2. Department of Neurology, Guizhou Aerospace Hospital, Zunyi 563000, China

Online:2013-07-15 Published:2013-06-28
Contact: DAI Zhi-kai1
Supported by:
广西高校优秀人才资助计划项目(桂教人[2009]62号)

摘要/Abstract

摘要：

目的：观察刺梨三萜(Rosa roxburghii Tratt triterpene，RRTT)对人肝癌细胞SMMC-7721细胞增殖的影响，研究其可能的作用机制。方法：采用形态学观察和MTT法分析RRTT对SMMC-7721细胞增殖的影响；通过NBT还原实验和细胞培养上清液中甲胎蛋白(alpha-fetoprotein，AFP)含量的测定分析RRTT对SMMC-7721分化的影响；采用 AO/EB染色法和FCM检测RRTT对肿瘤细胞周期和细胞凋亡的影响；RT-PCR检测Bad mRNA的表达。结果：RRTT 对SMMC-7721细胞增殖的抑制作用呈时间和剂量依赖性。与阴性对照组比，随RRTT剂量的增加，NBT阳性细胞比率增加，AFP含量逐渐降低。RRTT对SMMC-7721细胞凋亡和增殖周期均无明显影响。RRTT作用后，Bad mRNA的表达下调。结论：RRTT具有体外抗SMMC-7721作用，其机制可能通过下调Bad mRNA的表达而诱导细胞分化，而与抑制细胞增殖和诱导细胞凋亡无关。

关键词: 刺梨, 三萜化合物, 人肝癌SMMC-7721细胞, 细胞增殖, 细胞分化, 细胞凋亡

Abstract:

Objective: To explore the proliferative effect and potential mechanisms of Rosa roxburghii Tratt triterpene (RRTT)
on human hepatoma SMMC-7721 cells. Methods: Morphological changes, growth curves and MTT assay were used to
evaluate the anti-proliferative effect of RRTT on SMMC-7721 cells. Detection of alpha-fetoprotein (AFP) level in cultured
medium supernatant and NBT reduction assay were carried out to evaluate the inducive effect of RRTT on the differentiation
of SMMC-7721 cells. Cell apoptosis and cell cycle were examined by AO/EB fluorescent staining and flow cytometry.
SMMC-7721 cells treated with RRTT were collected for Bad transcript analysis by real-time reverse transcriptase–polymerase
chain reaction (RT-PCR). Results: The inhibitory effect of RRTT on the growth of SMMC-7721 cells tended to be in
RRTT dose-dependent and time-dependent manners. The ratio of NBT positive cells revealed an obvious increase and AFP
concentration of cultured medium exhibited a decline with increasing RTT dose when compared with the control. No obvious
difference in apoptosis or cell cycle distribution of SMMC-7721 cells was observed between the RRTT group and control
group. Relative mRNA level of Bad in SMMC-7721 cells was down-regulated by RRTT. Conclusion: RRTT has anticancer
effect on SMMC-7721 cells in vitro. Its possible mechanism is due to the cytoxicity and induction on cell differentiation via
Bad mRNA down-regulation, rather than the apoptosis and inhibition of cell proliferative cycle.

Key words: Rosa roxburghii Tratt, triterpene, human hepatoma SMMC-7721, cell proliferation, cell differentiation, apoptosis

中图分类号:

R730.5

黄姣娥1，江晋渝2，罗勇2，戴支凯1,*. 刺梨三萜对人肝癌SMMC-7721细胞增殖的影响[J]. 食品科学, doi: 10.7506/spkx1002-6630-201313058.

HUANG Jiao-e1，JIANG Jin-yu2，LUO Yong2，DAI Zhi-kai1,*. Effect of Rosa roxburghii Tratt Triterpene on Proliferation of Human Hepatoma SMMC-7721 Cells[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201313058.

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刺梨三萜对人肝癌SMMC-7721细胞增殖的影响

Effect of Rosa roxburghii Tratt Triterpene on Proliferation of Human Hepatoma SMMC-7721 Cells

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