食品科学 ›› 2021, Vol. 42 ›› Issue (17): 163-169.doi: 10.7506/spkx1002-6630-20200828-384

• 营养卫生 • 上一篇    下一篇

刺梨多酚对急性酒精中毒大鼠的解酒和护肝作用

周宏炫,黄颖,谭书明,涂永丽,罗继伟   

  1. (贵州大学酿酒与食品工程学院,贵州省农畜产品贮藏与加工重点实验室,贵州 贵阳 550025)
  • 发布日期:2021-09-29
  • 基金资助:
    贵州省农业重大产业科学研究攻关项目(黔教合KY字[2019]008)

Anti-alcoholic and Hepatoprotective Effects of Polyphenols from the Fruit of Rosa roxburghii Tratt. in Rats with Acute Alcoholism

ZHOU Hongxuan, HUANG Ying, TAN Shuming, TU Yongli, LUO Jiwei   

  1. (Guizhou Province Key Laboratory of Agricultural and Animal Products Processing & Storage, School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China)
  • Published:2021-09-29

摘要: 目的:研究刺梨多酚(Rosa roxburghii Tratt. polyphenols,RRTP)对急性酒精中毒大鼠的解酒和护肝作用。方法:将大鼠随机分为正常组、模型组、阳性对照组(海王金樽,100 mg/(kg mb·d))以及RRTP低、中、高剂量组(50、100、200 mg/(kg mb·d)),连续灌胃30 d后,除正常组给予生理盐水外,其余各组均灌胃15 mL/kg mb白酒(乙醇体积分数56%)建立急性酒精中毒模型。分别于酒后30、60、90、120 min剪尾取血,测定血液乙醇质量浓度;酒后12 h取血与肝脏,测定大鼠血清中谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)和甘油三酯(triglyceride,TG)水平,测定肝脏中乙醇代谢酶活力和抗氧化指标水平,并进行苏木精-伊红染色观察肝脏病理学变化;实时荧光定量聚合酶链式反应测定肝组织核因子E2相关因子2(nuclear factor erythroid-2 related factor 2,Nrf2)和血红素加氧酶-1(heme oxygenase-1,HO-1)mRNA相对表达水平。结果:与模型组相比,RRTP各剂量组均能降低大鼠血液乙醇、ALT、AST、TG水平和肝脏丙二醛含量,提高乙醇脱氢酶、乙醛脱氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶活力和谷胱甘肽含量,上调Nrf2、HO-1 mRNA表达量,并改善肝组织病理情况。结论:RRTP对急性酒精中毒大鼠具有较好的解酒和护肝作用,可能与其加快乙醇代谢和增强机体抗氧化能力有关。

关键词: 刺梨多酚;急性酒精中毒;解酒作用;护肝作用

Abstract: Objective: To study the anti-alcoholic and hepatoprotective effects of polyphenols from the fruit of Rosa roxburghii Tratt. (RRTP) in rats with acute alcoholism (AAH). Methods: Rats were randomly assigned to six groups: normal, model, positive control (Haiwangjinzun, a commercial dietary supplement with hepatoprotective potency; 100 mg/(kg mb·d)), and low-, medium- and high-dose RRTP (50, 100 and 200 mg/(kg mb·d)). After oral administration for 30 days, all rats except those in the normal group were administered by gavage with Chinese Baijiu with 56% (V/V) alcohol at a dose of 15 mL/kg mb to establish an AAH model. Instead, physiological saline was given to the normal group. At 30, 60, 90 and 120 min after administration of Baijiu, blood was collected from the tail vein to detect its alcohol concentration. At 12 h post-administration, blood and liver samples were collected for the detection of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and triglyceride (TG) levels in serum as well as the activities of ethanol metabolism-related enzymes and antioxidant enzymes in liver. Hematoxylin-eosin staining was performed to observe pathological changes of liver tissues. The?relative?mRNA?expression?levels?of?nuclear factor erythroid-2 related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were?measured by?real-time fluorescent quantitative polymerase chain reaction (PCR). Results: The levels of alcohol, ALT, AST and TG in serum and the level of malondialdehyde (MDA) in liver were decreased in the RRTP treatment groups when compared with the model group, while the activities of alcohol dehydrogenase, acetaldehyde dehydrogenase, superoxide dismutase, catalase and glutathione peroxidase and the content of glutathione were increased. Moreover, RRTP upregulated the relative mRNA expression levels of Nrf2 and HO-1 and ameliorated?hepatic pathological changes. Conclusion: In AAH rats, RRTP exhibits anti-alcoholic and hepatoprotective effects, and the underlying mechanism may be related to accelerating alcohol metabolism and enhancing antioxidant function.

Key words: polyphenols from the fruit of Rosa roxburghii Tratt.; acute alcoholism; anti-alcoholic effect; hepatoprotective effect

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