不同温度对无水保活条件下的中华鳖肌肉营养成分及血液生化指标影响

doi:10.7506/spkx1002-6630-201406042

食品科学 ›› 2014, Vol. 35 ›› Issue (6): 194-199.doi: 10.7506/spkx1002-6630-201406042

不同温度对无水保活条件下的中华鳖肌肉营养成分及血液生化指标影响

何蓉，谢晶*，苏辉，黎柳，黄硕琳

上海海洋大学食品学院，上海水产品加工及贮藏工程技术研究中心，上海 201306

收稿日期:2013-05-19 修回日期:2014-03-12 出版日期:2014-03-25 发布日期:2014-04-04
通讯作者: 谢晶 E-mail:jxie@shou.edu.cn
基金资助:
“十二五”国家科技支撑计划项目（2012BAD38B04）；上海市科委农业重点项目（11dz1960400）；
上海市科委工程中心建设项目（11DZ2280300）；上海高校一流学科建设计划项目

Effect of Temperature on Muscle Nutritional Components and Blood Biochemical Parameters of
Pelodiscus sinensis Alive without Water

HE Rong, XIE Jing*, SU Hui, LI Liu, HUANG Shuo-lin

Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center,
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China

Received:2013-05-19 Revised:2014-03-12 Online:2014-03-25 Published:2014-04-04
Contact: Jing XIE E-mail:jxie@shou.edu.cn

摘要/Abstract

摘要：

设定4、10 ℃和18 ℃组研究中华鳖（Pelodiscus sinensis）在无水保活21 d中质量损失率、肌肉水分、脂肪、蛋白质以及血液葡萄糖（Glu）、总蛋白（TP）、白蛋白（ALB）、尿素、尿酸（UA）和肌酐（CREA ）含量与乳酸脱氢酶（LDH）、碱性磷酸酶（AL P）和谷草转氨酶（AST）活性的变化。结果表明：在保活过程中， 4 ℃组中华鳖仅能存活11～16 d，其质量损失率、肌肉营养物质消耗量显著低于10 ℃和18 ℃组（P＜0.05），而血液Glu、LD H、AST、UA、CREA 水平均显著高于10 ℃和18 ℃组（P＜0.05）；18 ℃组质量损失率、肌肉营养物质消耗量、血液尿素浓度显著高于4 ℃和10 ℃组（P＜0.05）；10 ℃组肌肉营养物质消耗量及血液生理指标水平间于 4 ℃和18 ℃组之间；随着保活时间的推移，3 个温度组肌肉营养物质和血液TP、ALB、ALP水平均呈现下降趋势，而质量损失率和血液尿素、UA水平均呈现上升趋势；4 ℃组肌肉ATP和血液LDH、AST、CREA水平均呈现先升高后下降的趋势；10 ℃和18 ℃组肌肉ATP含量呈现先下降后上升的趋势，而血液AST活性呈现下降趋势。该实验结果表明，4 ℃组中华鳖的营养物质损害量低，但低温应激对该组中华鳖新陈代谢水平和生命活力产生负面影响， 18 ℃组中华鳖机体能量代谢水平旺盛，营养物质消耗量和质量损失率均较大，不利于实现保活价值。建议将中华鳖保活温度设定为10 ℃左右。

关键词: 中华鳖, 温度, 营养物质, 生化指标

Abstract:

Pelodiscus sinensis was kept alive without water by incubation in a thermostatic chamber set at different
temperatures (4, 10 and 18 ℃) for 21 days. The changes in body weight loss, the contents of water, lipid and protein in
muscle, and the concentrations of glucose (Glu), total protein (TP), albumin (ALB), urea, uric acid (UA) and creatinine
(CREA) as well as the activities of lactate dehydrogenase (LDH), alkaline phosphatase (AL P) and aspartate aminotransferase
(AST) in blood were examined during the incubation period. The results indicated that the survival time of Pelodiscus
sinensis at 4 ℃ was 11–16 days. The body weight loss and nutrient consumption of Pelodiscus sinensis at this temperature
were lower than those observed at 10 and 18 ℃ (P ＜ 0.05) and the concentrations of Glu, UA and CREA , and the
activities of LDH and AST were also higher (P ＜ 0.05). The body weight loss, nutrient consumption and blood urea
concentration of Pelodiscus sinensis at 18 ℃ were higher than those observed at 4 and 10 ℃ (P ＜ 0.05). Additionally, the
nutrient consumption and blood physiological indexes of Pelodiscus sinensis at 10 ℃ were between those at 4 and 18 ℃.
The nutrition, blood TP and ALB levels and ALP activity of Pelodiscus sinensis at three different temperatures showed a
decreasing trend during the incubation period, while the body weight loss, and urea and UA levels exhibited an increasing
trend. The levels of ATP, LD H, AST and CREA in Pelodiscus sinensis at 4 ℃ increased initially and then decreased.
Furthermore, ATP content of Pelodiscus sinensis at 10 and 18 ℃ exhibited an initial decrease followed by a increase,
while AST activity always decreased. These results suggest that the nutrient consumption of Pelodiscus sinensis at 4 ℃ is
lower, while low temperature stress has a harmful effect on the metabolism and vitality of Pelodiscus sinensis. The body
metabolism rate of Pelodiscus sinensis at 18 ℃ is faster and the nutrient consumption and body weight rate are higher.
Therefore, the most appropriate temperature for keeping Pelodiscus sinensis alive is about 10 ℃.

Key words: Pelodiscus sinensis, temperature, nutrition, biochemical parameters

中图分类号:

TS254.4

何蓉，谢晶*，苏辉，黎柳，黄硕琳. 不同温度对无水保活条件下的中华鳖肌肉营养成分及血液生化指标影响[J]. 食品科学, 2014, 35(6): 194-199.

HE Rong, XIE Jing*, SU Hui, LI Liu, HUANG Shuo-lin. Effect of Temperature on Muscle Nutritional Components and Blood Biochemical Parameters of
Pelodiscus sinensis Alive without Water[J]. FOOD SCIENCE, 2014, 35(6): 194-199.

参考文献

[1] 何蓉, 谢晶. 水产品保活技术研究现状和进展[J]. 食品与机械, 2012, 28(5): 243-246.

[2] Carlos A, Palacios M, Tovar E B, Taylor J F. Effect of temperature on growth and survival of Chirostoma estor, Jordan 1879, monitored using a simple video technique for remote measurement of length and mass of larval and juvenile fishes. Aquaculture, 2002(209): 369-377.

[3] 邱伟强, 陈刚, 陈瞬胜等. 离子对反相高效液相色谱法同时检测水产品中6种ATP关联化合物[J]. 水产学报, 2011, 35(11):1745-1751.

[4] 廖晓霞, 叶均安. 中华鳖的消化道指数和血液生化指标[J]. 上海水产大学学报, 2006, 15(1): 105-108.

[5] 周玉, 郭文场, 杨振国等. 鱼类血液学指标研究的进展[J].上海水产大学学报, 2001, 10(2): 163-165.

[6] 孙学亮, 邢克智, 陈成勋等. 急性温度胁迫对半滑舌鳎血液指标的影响[J]. 水产科学, 2010, 29(7): 387-392.

[7] 李秀峰.低温胁迫下东北林蛙血糖和相关酶的探究[D].东北林业大学, 哈尔滨, 2012.

[8] 刘云, 杨小林, 王朝莉. 中华鳖冬眠期与活动期部分生理生化性质的比较研究[J]. 四川大学学报, 2002, 39(S1): 82-84.

[9] Lukyanenko V I, Khabarov M V, Lukyanenko V V. Heterogeneity and polymorphism of functionally specialized blood proteins in migratory fish: The case study of the north cas pianpopulation of the russiansturgeonduring sea andriver periods of life. 1. Albumins. Biology Bulletin, 2002(29): 293-299.

[10] Mommsen T P, French C J, Hochachka P W. Sites and patterns of protein and amino acid utilization during the spawning migration of salmon[J]. Canadian Journal of Zoology, 1980, 58(10): 1785-1799.

[11] 谢小军, 邓利, 张波. 饥饿对鱼类生理生态学影响的研究进展[J]. 水生生物学报. 1988, 22(2): 181-187.

[12] 李永才, 黄溢明. 比较生理学[M ]. 北京: 高等教育出版社. 1993, 123-134.

[13] 蒋春琴, 冷向军, 李小勤等. 醋酸棉酚对异育银鲫生长性能血清生化指标和棉酚残留的影响[ J ]. 动物营养学报, 2012, 24(1): 168-175.

[14] 华雪铭, 朱站英, 邢思华等. 饥饿对草鱼非特异免疫水平的影响[J]. 动物学杂志. 2012, 47(2): 91-97.

[15] Jyothi B, Narayan G. Effect of phorate on certain protein profiles of serum in freshwater fish, Clarias batrachus Linn. [ J]. Journal of Environmental Biology, 1997(18): 137-140.

[16] 崔杰峰, 潘柏申. 急性心肌梗死血清酶标志物沿革[J]. 上海医学检验杂志. 2000, 15(1): 13-14.

[17] 林仕梅, 罗莉, 叶元士. 喹乙醇对草鱼几项生理指标的影响[J]. 西南农业学报, 1998, 11(2): 119-123.

[18] 钱云霞, 陈惠群, 孙江飞. 饥饿对养殖鲈鱼血液生理生化指标的影响[J]. 中国水产科学. 2002, 9(2): 133-136.

[19] 杨成辉, 蔡勋, 刘霞等. 饥饿和再投喂对哲罗鱼幼鱼血液生理生化指标的影响[J]. 淡水渔业. 2009, 39(1): 36-40.

[20] Li S Z, Yuan X J, Yang Y Y, et al. Effect of cold stress by immersing in water on some enzyme activities and digestive tract mucosal hyperemia inchicrken[J]. Chin J Appl EnvironBiol, 2001, 7(3) :267-270.

[21] Flavia S R, Gilberto M, Eliane T O, et al. Mobilization and recovery of energy stores in traira, Hoplias malabaricus Bloch (Teleostei, Erythrinidae) during long-term starvation and after re-feeding[J]. Journal of Comparative Physiology, 2006, 176(7): 721-728.

[22] 刘伟东, 薛长湖, 殷邦忠等. 低温下大菱鲆有水和无水保活过程中生理生化变化的研究[J]. 渔业科学进展. 2009, 30(5): 69-74.

不同温度对无水保活条件下的中华鳖肌肉营养成分及血液生化指标影响

Effect of Temperature on Muscle Nutritional Components and Blood Biochemical Parameters of
Pelodiscus sinensis Alive without Water

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不同温度对无水保活条件下的中华鳖肌肉营养成分及血液生化指标影响

Effect of Temperature on Muscle Nutritional Components and Blood Biochemical Parameters of Pelodiscus sinensis Alive without Water

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Effect of Temperature on Muscle Nutritional Components and Blood Biochemical Parameters of
Pelodiscus sinensis Alive without Water