黑灵芝多糖对Ⅱ型糖尿病大鼠肝脏氧化应激损伤的保护作用

doi:10.7506/spkx1002-6630-201321060

食品科学 ›› 2013, Vol. 34 ›› Issue (21): 302-305.doi: 10.7506/spkx1002-6630-201321060

黑灵芝多糖对Ⅱ型糖尿病大鼠肝脏氧化应激损伤的保护作用

林素丽，朱科学，宋丹，聂少平*，谢明勇

南昌大学食品科学与技术国家重点实验室，江西南昌 330047

收稿日期:2013-06-24 修回日期:2013-10-08 出版日期:2013-11-15 发布日期:2013-10-28
通讯作者: 聂少平 E-mail:spnie@ncu.edu.cn
基金资助:
国家自然科学基金项目(31071532)；新世纪优秀人才支持计划项目(NCET-12-0749)；
“十二五”国家科技支撑计划项目(2012BAD33B06)；南昌大学大学生创新训练项目(2012047)

Protective Effect of Polysaccharide from Ganoderma atrum against Oxidative Stress-induced Liver Injury in Type Ⅱ Diabetic Rats

LIN Su-li，ZHU Ke-xue，SONG Dan，NIE Shao-ping*，XIE Ming-yong

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China

Received:2013-06-24 Revised:2013-10-08 Online:2013-11-15 Published:2013-10-28
Contact: Shao-Ping NIE E-mail:spnie@ncu.edu.cn

摘要/Abstract

摘要：

目的：研究黑灵芝多糖对Ⅱ型糖尿病大鼠肝脏氧化应激损伤的保护作用。方法：高脂高糖结合小剂量链脲佐菌素(STZ)诱导Ⅱ型糖尿病大鼠动物模型，挑选成模的糖尿病大鼠，随机分为3组(每组8只)：糖尿病对照组、黑灵芝多糖组(100mg/(kg·d))，N-乙酰-L-半胱氨酸(NAC)组(100mg/(kg·d))。给药4周后，检测并分析各组大鼠肝功能指标、肝脏器官指数和肝糖原含量以及肝脏抗氧化指标，并利用RT-PCR法测定肝脏组织Bax、Bcl-2 mRNA的表达。结果：黑灵芝多糖(100mg/(kg·d))能够使Ⅱ型糖尿病大鼠肝脏指数显著降低，肝糖原含量显著增加，肝功能指标谷草转氨酶(AST)、谷丙转氨酶(ALT)趋于正常，超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性显著增加，丙二醛(MDA)含量显著降低。此外，黑灵芝多糖还能促进Bcl-2蛋白的表达，抑制Bax蛋白的表达。结论：黑灵芝多糖(100mg/(kg·d))对Ⅱ型糖尿病大鼠肝脏氧化应激损伤具有保护作用。

关键词: 黑灵芝多糖, 氧化应激, Ⅱ型糖尿病, 抗氧化, Bax, Bcl-2

Abstract:

Objective: To explore the protective effect of polysaccharide from Ganoderma atrum against oxidative stressinduced
liver injury in type Ⅱdiabetic rats. Methods: An animal model of type Ⅱ diabetes was developed by feeding high
fat diet to rats coupled with an injection of STZ at low dose (30 mg/(kg·d)). Type Ⅱ diabetic rats were randomly divided
into three groups: diabetic control group, polysaccharide-treated (100 mg/(kg·d)) diabetic group and NAC (N-acetyl-Lcysteine,
100 mg/(kg·d))-treated diabetic group. Eight normal rats fed on standard normal chow diet served as a non-diabetic
control group. At the end of 4 weeks experimental period, liver function (ALT and AST), liver index, liver glycogen
and antioxidant enzyme activities (SOD, GSH-Px and CAT) as well as lipid peroxidation (MDA) were measured. Bax
and Bcl-2 mRNA expression levels were analyzed by RT-PCR. Results: Ganoderma atrum polysaccharide significantly
improved liver function and liver index, and also increased glycogen content and antioxidant enzyme activities as well as
decreased MDA content in liver of diabetic rats. Moreover, Bcl-2 expression was promoted whereas Bax expression was
inhibited. Conclusion: Polysaccharide from Ganoderma atrum (100 mg/(kg·d)) may improve the liver function of type
Ⅱ diabetic rats through antioxidant effect.

Key words: polysaccharide from Ganoderma atrum, oxidative stress, type Ⅱ diabetes, antioxidant, Bax, Bcl-2

中图分类号:

R575.5

林素丽，朱科学，宋丹，聂少平*，谢明勇. 黑灵芝多糖对Ⅱ型糖尿病大鼠肝脏氧化应激损伤的保护作用[J]. 食品科学, 2013, 34(21): 302-305.

LIN Su-li，ZHU Ke-xue，SONG Dan，NIE Shao-ping*，XIE Ming-yong. Protective Effect of Polysaccharide from Ganoderma atrum against Oxidative Stress-induced Liver Injury in Type Ⅱ Diabetic Rats[J]. FOOD SCIENCE, 2013, 34(21): 302-305.

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黑灵芝多糖对Ⅱ型糖尿病大鼠肝脏氧化应激损伤的保护作用

Protective Effect of Polysaccharide from Ganoderma atrum against Oxidative Stress-induced Liver Injury in Type Ⅱ Diabetic Rats

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