关键词: 北五味子木脂素, 肝损伤, 酒精, 氧化应激

Abstract: Objective To explore the protective effect of Schisandra lignans (SCL) on acute liver injury induced by ethanol in mice and to reveal its mechanism. Methods 50 mice were randomly divided into normal control group, model group, low-dose SCL group (25 mg/kg), high-dose SCL group (50 mg/kg) and positive control group (bifendate, 150 mg/kg). The corresponding agents were intragastrically administrated to all the mice respectively for 15 days. 50% ethanol (12.0 mL/kg) was given to the mice in model groups 1 hour after the last administration. The mice of normal control group were administrated match volume distilled water. All the mice were sacrificed 12 hours later, and their blood samples were collected. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, triglyceride (TG) level in serum, malondialdehyde (MDA) and reduced glutathione (GSH) contents, and nitric oxide synthase (NOS) activity in liver tissues were measured. HE staining was performed for observing pathological changes of the liver tissues. Results SCL could significantly decrease the ALT, AST activities and TG levels in serum（P＜0.05）. Meanwhile the MDA contents and NOS activities in liver tissues were reduced significantly, and the GSH contents in liver tissues were increased by SCL（P＜0.05）. Moreover, HE staining showed that SCL could significantly improve the liver cell edema, necrosis, and central venous congenstion. Conclusion Schisandra lignans has a significant protective effect on alcohol-induced acute liver injury, which might be related to the anti-oxidation.

Key words: Schisandra lignans, liver injury, alcohol, oxidative stress