应用脂肪酸甲酯快速筛选植物油中掺假地沟油

doi:10.7506/spkx1002-6630-201416029

食品科学

应用脂肪酸甲酯快速筛选植物油中掺假地沟油

赵海香1，任荷玲2，许秀丽2，张凤霞2，丁罡斗2，乐粉鹏2，马小宁2，仲维科2,*

1.河北北方学院应用化学研究所，河北张家口 075000；2.中国检验检疫科学研究院，北京 100123

出版日期:2014-08-25 发布日期:2014-08-25
通讯作者: 仲维科
基金资助:
质检总局“双打”专项基金项目（2012104010-1）

Rapid Screening for Gutter Oil Adulteration in Vegetable Oil Based on Fatty Acid Methyl Esters

ZHAO Hai-xiang1, REN He-ling2, XU Xiu-li2, ZHANG Feng-xia2, DING Gang-dou2, YUE Fen-peng2, MA Xiao-ning2, ZHONG Wei-ke2,*

1. Institute of Applied Chemistry, Hebei North University, Zhangjiakou 075000, China;
2. Chinese Academy of Inspection and Quarantine, Beijing 100123, China

Online:2014-08-25 Published:2014-08-25
Contact: ZHONG Wei-ke

摘要/Abstract

摘要：

根据植物油和地沟油中脂肪酸酯存在方式的不同，建立植物油中地沟油掺假的快速筛选方法。植物油中脂肪酸是以甘油三酯的形式存在，地沟油中脂肪酸以脂肪酸甲酯的形式存在，利用气相色谱-质谱的全扫描模式，结合NIST 05标准谱库检索功能，对37 种脂肪酸甲酯进行测定。样品采用直接稀释-气相色谱-质谱全扫描分析脂肪酸甲酯，100 个植物油样品中均未检出脂肪酸甲酯；20 个地沟油样品中13 个检出肉豆蔻酸甲酯（C14∶0）、棕榈酸甲酯（C16∶0）和硬脂酸甲酯（C18∶0）等14 种脂肪酸甲酯，检出率为65%，且70%的检出脂肪酸甲酯的地沟油样品中脂肪酸甲酯种类多、含量较高。因此，若植物油样品脂肪酸甲酯种类多、含量高，则可判定为有地沟油掺假；若脂肪酸甲酯含量低或没有脂肪酸甲酯，则可以进一步用其他指标和方法进行检测，脂肪酸甲酯的检测方法可以作为地沟油掺假的快速粗筛方法。

关键词: 脂肪酸甲酯, 植物油, 地沟油, 气相色谱-质谱

Abstract:

A rapid screening method for adulteration of gutter oil in vegetable oils based on different forms of fatty acid
esters detected in samples was developed. The major forms of fatty acids were triglycerides in vegetable oils and fatty acid
methyl esters (FAMEs) in gutter oil. Using gas chromatography-mass spectrometry (GC-MS) in a full-scanning detection
mode, 37 kinds of fatty acid methyl esters were determined by searching against the NIST 05 standard mass spectral library.
The fatty acid methyl esters of samples were determined by direct dilution with n-hexane and GC-MS with full-scanning
analysis. Fatty acid methyl esters were not detected in 100 vegetable oil samples. Meanwhile, 14 kinds of fatty acid methyl
esters were detected in 13 (65%) out of 20 gutter oil samples, mainly including methyl myristate (C14∶0), methyl palmitate
(C16∶0) and methyl stearate (C18∶0). In addition, 70% of the 13 gutter samples were detected to simultaneously contain many
kinds of fatty acid methyl esters at higher levels. Thus, vegetable oil sample detected to contain more fatty acid methyl esters
at higher contents can be confirmed to be adulterated with gutter oil. In the case of lower or undetectable levels of fatty acid
methyl esters, other indicators or methods are needed. In conclusion, fatty acid methyl esters can be used an indicator for
rapid screening for gutter oil adulteration in vegetable oils.

Key words: fatty acid methyl esters (FAMEs), vegetable oil, gutter oil, gas chromatography-mass spectrometry (GC-MS)

中图分类号:

TS207.5

赵海香1，任荷玲2，许秀丽2，张凤霞2，丁罡斗2，乐粉鹏2，马小宁2，仲维科2,*. 应用脂肪酸甲酯快速筛选植物油中掺假地沟油[J]. 食品科学, doi: 10.7506/spkx1002-6630-201416029.

ZHAO Hai-xiang1, REN He-ling2, XU Xiu-li2, ZHANG Feng-xia2, DING Gang-dou2, YUE Fen-peng2, MA Xiao-ning2, ZHONG Wei-ke2,*. Rapid Screening for Gutter Oil Adulteration in Vegetable Oil Based on Fatty Acid Methyl Esters[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201416029.

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应用脂肪酸甲酯快速筛选植物油中掺假地沟油

Rapid Screening for Gutter Oil Adulteration in Vegetable Oil Based on Fatty Acid Methyl Esters

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