食品科学 ›› 0, Vol. ›› Issue (): 0-0.

• 成分分析 •    下一篇

3种名优绿茶特征香气成分的比较及种类判别分析

龙立梅1,宋沙沙2,李 柰2,樊琛2,李小波2,曹学丽1   

  1. 1. 北京工商大学食品学院
    2. 北京工商大学
  • 收稿日期:2014-06-28 修回日期:2014-11-25 出版日期:2015-01-25 发布日期:2015-01-16
  • 通讯作者: 龙立梅 E-mail:longlimei112@sohu.com
  • 基金资助:
    国家质量监督检验检疫总局公益性行业科研专项

Comparisons for characteristic aroma components and cultivar discriminant analysis of three varieties of famous green tea

  • Received:2014-06-28 Revised:2014-11-25 Online:2015-01-25 Published:2015-01-16
  • Contact: Li-Mei LONG E-mail:longlimei112@sohu.com

摘要: 采用顶空固相微萃取与气相色谱-质谱联用技术,建立绿茶香气分析方法,研究固相微萃取温度和萃取时间对绿茶香气物质种类和总量的影响,并对西湖龙井、黄山毛峰和信阳毛尖3种名优绿茶特征香气成分进行分析研究和种类判别。结果表明:采用50/30μm DVB/CAR/PDMS萃取头,在80℃下吸附 60min,能达到最佳吸附效果;通过对三种绿茶特征香气组分分析,分析鉴定出58种绿茶特征香气成分,相对含量较高的主要成分依次为:香叶醇、(Z)-己酸-3-己烯酯、芳樟醇、壬醛、反式-橙花叔醇等。利用16个共有特征香气成分峰的相对峰面积,建立多元化典型判别函数,采用逐步判别分析技术对31个绿茶样品进行了很好的种类判别,判别正确率近96.8%。

关键词: 顶空固相微萃取, 气相色谱-质谱法, 绿茶, 香气成分, 种类判别

Abstract: In order to establish a method for determining the aroma components of green tea, headspace solid phase micro-extraction (HS-SPME) was coupled to gas chromatography-mass spectrometry (GC-MS). The effects of SPME temperature and adsorption times on the kinds and amounts of aroma compounds of green tea were evaluated.The characteristic aroma components were researched and varieties were discriminated analysis of three famous quality green tea,including Xihu Longjing,Huangshan Maofeng and Xinyang Maojian. The results showed that Optimum adsorption was achieved using 50/30 μm DVB-CAR-PDMS fiber for adsorption at 80 ℃ for 60min. A total of 58 characteristic aroma compounds were found in the three kinds of green tea. The main components of the relative content are higher in the order: Geraniol, 3-hexenyl ester, (Z)-Hexanoic acid, Linalool, 1-Nonanal, Nerolidol,et al. When diversified typical discriminant functions were established using the relative peak area of 16 common characteristic aroma compounds peak. Using stepwise discriminant analysis technology to 31 samples of green tea had good varieties, discriminant accuracy of nearly 96.8%.

Key words: headspace solid phase micro-extraction (HS-SPME), gas chromatography-mass spectrometry (GC-MS), green tea, aroma components, variety discriminant

中图分类号: