食品科学

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基于液相色谱-质谱联用技术检测食物过敏原研究进展

熊丽姬,佟 平,肖 娜,陈红兵   

  1. 1.南昌大学 食品科学与技术国家重点实验室,江西 南昌 330047;
    2.南昌大学环境与化学工程学院,江西 南昌 330047;3.南昌大学中德联合研究院,江西 南昌 330047
  • 出版日期:2014-11-15 发布日期:2014-11-06

Progress in Detection of Food Allergens Based on Liquid Chromatography Coupled with Mass Spectrometry

XIONG Li-ji, TONG Ping, XIAO Na, CHEN Hong-bing   

  1. 1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;
    2. School of Environment and Chemical Engineering, Nanchang University, Nanchang 330047, China;
    3. Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China
  • Online:2014-11-15 Published:2014-11-06

摘要:

食物过敏是目前重要的食品安全问题,检测食物过敏原对保护过敏患者具有重要意义。检测食物基质中痕量的过敏成分依赖于可靠的分析方法。液相色谱-质谱联用技术具有高灵敏性、高分辨率、高自动化等特点,被广泛应用于食物过敏原蛋白质的研究中。本文主要阐述了液相色谱-质谱联用技术在单一过敏食物中过敏原蛋白质的检测方面和在复杂基质中多个食物过敏原蛋白质的检测方面的应用,并简要概述了提高液相色谱-质谱联用技术检测过敏原蛋白精确度的措施,指出液相色谱-质谱联用技术在检测食物过敏原方面还需要继续深入研究。

关键词: 液相色谱-质谱联用, 食物过敏原, 检测

Abstract:

Food allergy is currently an important problem in food safety, and the detection of food allergens is of great
significance for protecting allergic consumers. The detection of trace allergenic proteins in a complex food matrix mainly
relies on a reliable analysis method. Liquid chromatography-mass spectrometry (LC-MS) with the advantages of high
throughput, high resolution and high automation is widely used to explore food allergen proteins. This article reviews the
application of LC-MS for detecting allergens in a single or complicated food matrix, and suggests ways to improve the
accuracy of LC-MS. However, further studies are necessary for the detection of allergens in food matrices by LC-MS.

Key words: liquid chromatography-mass spectrometry (LC-MS), food allergen, detection

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