食品科学

• 生物工程 • 上一篇    下一篇

浆水中微生物的分离与鉴定

李雪萍,李建宏,孟宪刚*,郑 楠,谢佳佳   

  1. 兰州交通大学化学与生物工程学院,甘肃 兰州 730070
  • 发布日期:2014-12-15

Isolation and Identification of Microorganisms from Jiangshui, a Traditional Chinese Fermented Vegetable Product

LI Xue-ping, LI Jian-hong, MENG Xian-gang*, ZHENG Nan, XIE Jia-jia   

  1. School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China
  • Published:2014-12-15

摘要:

以浆水为实验材料,在多种培养基平板上分离纯化其中的微生物,共得到24 株菌,其中细菌20 株,丝状真菌3 株,酵母菌1 株。对纯化后的各菌株进行菌落形态观察、革兰氏染色、生化特性研究以及分子生物学鉴定,结果表明:浆水中存在多种微生物,其细菌以乳杆菌属或双歧杆菌属等益生菌为主,酵母菌为酿酒酵母,丝状真菌为变灰青霉和橘青霉。结果说明浆水微生物组成优良,具有开发利用潜力,但其中还存在杂菌和有害菌,应在生产中引起进一步重视。

关键词: 浆水, 微生物, 分离, 鉴定

Abstract:

In this study, 24 strains were isolated from Jiangshui, a traditional Chinese fermented celery product, and purified
by various culture medium plates, including 20 strains of bacteria, 3 strains of filamentous fungi, and 1 strain of yeast. The
purified strains were analyzed through morphological, Gram staining, biochemical characteristics and molecular biology
identification. The results showed that various microorganisms existed in Jiangshui, and the bacteria mainly belonged to
the genera Lactobacillus or Bifidobacterium. The yeast was Saccharomyces cerevisiae, and the filamentous fungi were
Penicillium canescens and Penicillium citrinum. These results illustrate that the composition of microorganisms is excellent,
with a good potential for development and utilization. But there are also some miscellaneous bacteria and harmful bacteria,
which should be taken seriously in production.

Key words: Jiangshui, microorganism, isolation, identification

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