酶法降解鸭肠中胆固醇对鸭肠品质的影响

doi:10.7506/spkx1002-6630-201507026

食品科学

酶法降解鸭肠中胆固醇对鸭肠品质的影响

丁顺杰，傅裕，钟雨珅，丁晓雯

西南大学食品科学学院，重庆市农产品加工及贮藏重点实验室，重庆 400715

出版日期:2015-04-15 发布日期:2015-05-05

Effect of Enzymatic Degradation of Cholesterolon Duck Intestine Quality

DING Shunjie, FU Yu, ZHONG Yushen, DING Xiaowen*

Chongqing Key Laboratory of Produce Processing and Storage, College of Food Science, Southwest University, Chongqing 400715, China

Online:2015-04-15 Published:2015-05-05

摘要/Abstract

摘要：

目的：研究酶法降解鸭肠中胆固醇对其品质的影响，为工业化生产低胆固醇鸭肠提供实验数据。方法：自制胆固醇氧化酶粗酶降解鸭肠中胆固醇；分光光度法测定胆固醇含量；质构剖面分析法（texture profileanalysis，TPA）和色差分析法测定鸭肠质地及色泽变化。结果：经粗酶处理的鸭肠胆固醇含量为26.31 mg/g，降解率为25.81%；挥发性盐基总氮（total volatile basic nitrogen，TVBN）含量为13.30 mg/100 g，pH 6.87，硬度为10 459.10 g，弹性为0.96，咀嚼性为6 816.36 g，鸭肠内、外壁亮度值分别为60.52、62.23。结论：自制胆固醇氧化酶粗酶可以有效降解鸭肠胆固醇，对其感官品质有一定的改善。

关键词: 鸭肠, 胆固醇氧化酶, 胆固醇, 感官品质

Abstract:

Objective: To explore the effect of enzymatic degradation of cholesterol on duck intestine quality. Methods:
Crude cholesterol oxidase was prepared in our laboratory and used to degrade cholesterol in duck intestine. Duck intestinal
cholesterol was measured by a spectrophotometric method. Duck intestine was subjected to textural profile analysis (TPA)
and color difference analysis. Results: After duck intestine was treated by cholesterol oxidase, its cholesterol content was
reduced by 25.81% to 26.31 mg/g; TVBN was 13.30 mg/100 g, pH was 6.87, hardness was 10 459.10 g, elasticity was
0.96, chewiness was 6 816.36 g, and the brightness of inner wall and outer wall of duck intestine were 60.52 and 62.23,
respectively. Conclusion: The cholesterol oxidase could effectively degrade duck intestinal cholesterol, thereby improving
the quality of duck intestine.

Key words: duck intestine, cholesterol oxidase, cholesterol, sensory quality

中图分类号:

TS201.3

丁顺杰，傅裕，钟雨珅，丁晓雯. 酶法降解鸭肠中胆固醇对鸭肠品质的影响[J]. 食品科学, doi: 10.7506/spkx1002-6630-201507026.

DING Shunjie, FU Yu, ZHONG Yushen, DING Xiaowen*. Effect of Enzymatic Degradation of Cholesterolon Duck Intestine Quality[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201507026.

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酶法降解鸭肠中胆固醇对鸭肠品质的影响

Effect of Enzymatic Degradation of Cholesterolon Duck Intestine Quality

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